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• W2061133991 abstract "Three different proteolytic enzymes could be identified in supernatants from cultures of Staphylococcus aureus, strain V8, if grown in a casein hydrolysate-yeast extract medium (CCYI). One protease was not produced if the yeast extract was omitted from the medium. This protease was inactivated by ethylene-diamino-tetra-acetic acid (EDTA, 5.0 mM at 20° C for 30 min). If calcium ions (final cone. 2.5 mM) were added to the yeast extract-free medium, the EDTA-sensitive protease was formed in amounts approaching those obtained in the complete medium. The formation of the EDTA-stable enzymes was not affected by calcium ions. The two EDTA-stable activities could be separated by isoelectric focusing, one with a pI of 4.0 and the other with a pI of 9.4. The formation of the EDTA-stable protease was stimulated by yeast extract." @default.
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• W2061133991 date "2009-08-15" @default.
• W2061133991 modified "2023-10-01" @default.
• W2061133991 title "THE FORMATION OF EXTRACELLULAR PROTEOLYTIC ENZYMES BY STAPHYLOCOCCUS AUREUS" @default.
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• W2061133991 doi "https://doi.org/10.1111/j.0365-5563.1973.tb00009.x" @default.
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