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- W2061150241 abstract "A method was developed for the simultaneous measurement of acetylene reduction, carbon dioxide evolution and oxygen uptake by individual root nodules of intact nitrogen-fixing plants (Alnus rubra Bong.). The nodules were enclosed in a temperature-controlled leak-tight cuvette. Assay gas mixtures were passed through the cuvette at a constant, known flow rate and gas exchange was measured by the difference between inlet and outlet gas compositions. Gas concentrations were assayed by a combination of an automated gas chromatograph and a programmable electronic integrator. Carbon dioxide and ethylene evolution were determined with a coefficient of variation which was less than 2%, whereas the coefficient of variation for oxygen uptake measurements was less than 5%. Nodules subjected to repeated removal from and reinsertion into the cuvette and to long exposures of 10% v/v acetylene showed no irreversible decline in respiration or acetylene reduction. This system offers long-term stability and freedom from disturbance artifacts plus the ability to monitor continuously, rapidly and specifically the changes in root nodule activity caused by environmental perturbation." @default.
- W2061150241 created "2016-06-24" @default.
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- W2061150241 date "1982-08-01" @default.
- W2061150241 modified "2023-09-25" @default.
- W2061150241 title "Simultaneous Measurement of Acetylene Reduction and Respiratory Gas Exchange of Attached Root Nodules" @default.
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- W2061150241 doi "https://doi.org/10.1104/pp.70.2.361" @default.
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