FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2061163368> ?p ?o ?g. }

• W2061163368 endingPage "1712" @default.
• W2061163368 startingPage "1700" @default.
• W2061163368 abstract "Previous studies showed decreased protein kinase C (PKC)-delta expression in azoxymethane-induced rat and sporadic human colonic tumors. To elucidate the role of PKC-delta on the neoplastic phenotype of human colon cancer cells, we established stable transfectants of this isoenzyme in CaCo-2 cells.Human PKC-delta complementary DNA was subcloned into 2 distinct metallothionein-regulated expression vectors. Polyclonal populations of PKC-delta transfectants were characterized by Western blotting. PKC-delta activity was measured in situ using a PKC-delta-specific substrate. Proliferation was determined by Coulter counter, and cell cycle distribution was analyzed by flow cytometry. In vitro transformation was assessed by growth in soft agar and differentiation by changes in alkaline phosphatase and sucrase isomaltase. Apoptosis was evaluated by 4',6-diamidino-2-phenylindole dihydrochloride and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling staining.In the presence of Zn(2+), PKC-delta transfectants expressed a 4-fold increase in the protein and a 2-fold increase in activity of PKC-delta. PKC-delta transfectants exhibited a 30% decrease (P < 0.05) in cell growth and an enhanced differentiation phenotype. Increased PKC-delta expression induced a significant G0/G1 arrest, inhibited anchorage-independent growth (50%, P < 0.05), and caused a 2-fold increase in apoptosis (P < 0.05).Our studies show that increased expression of PKC-delta inhibits anchorage-dependent and -independent growth, while inducing cellular differentiation and limiting survival of this human colon cancer cell line." @default.
• W2061163368 created "2016-06-24" @default.
• W2061163368 creator A5005112247 @default.
• W2061163368 creator A5030030043 @default.
• W2061163368 creator A5031508697 @default.
• W2061163368 creator A5035049656 @default.
• W2061163368 creator A5036288948 @default.
• W2061163368 creator A5058117904 @default.
• W2061163368 creator A5080181876 @default.
• W2061163368 date "2001-06-01" @default.
• W2061163368 modified "2023-10-10" @default.
• W2061163368 title "PKC-δ inhibits anchorage-dependent and -independent growth, enhances differentiation, and increases apoptosis in CaCo-2 cells" @default.
• W2061163368 cites W1502446689 @default.
• W2061163368 cites W1524612147 @default.
• W2061163368 cites W1538433900 @default.
• W2061163368 cites W1554882994 @default.
• W2061163368 cites W1559244741 @default.
• W2061163368 cites W1572814792 @default.
• W2061163368 cites W1576671418 @default.
• W2061163368 cites W1935588396 @default.
• W2061163368 cites W1972491475 @default.
• W2061163368 cites W1978265027 @default.
• W2061163368 cites W1986032509 @default.
• W2061163368 cites W1987534522 @default.
• W2061163368 cites W1989706319 @default.
• W2061163368 cites W1990026949 @default.
• W2061163368 cites W1995466641 @default.
• W2061163368 cites W1995501320 @default.
• W2061163368 cites W2012345358 @default.
• W2061163368 cites W2015571904 @default.
• W2061163368 cites W2018297481 @default.
• W2061163368 cites W2018801742 @default.
• W2061163368 cites W2020428264 @default.
• W2061163368 cites W2022190981 @default.
• W2061163368 cites W2034684462 @default.
• W2061163368 cites W2035503012 @default.
• W2061163368 cites W2048766756 @default.
• W2061163368 cites W2062077798 @default.
• W2061163368 cites W2064229297 @default.
• W2061163368 cites W2064355969 @default.
• W2061163368 cites W2066909100 @default.
• W2061163368 cites W2070716283 @default.
• W2061163368 cites W2080275316 @default.
• W2061163368 cites W2082000305 @default.
• W2061163368 cites W2106739551 @default.
• W2061163368 cites W2111133130 @default.
• W2061163368 cites W2117250040 @default.
• W2061163368 cites W2123927174 @default.
• W2061163368 cites W2126415875 @default.
• W2061163368 cites W2133991390 @default.
• W2061163368 cites W2138302361 @default.
• W2061163368 cites W2140905337 @default.
• W2061163368 cites W2151630930 @default.
• W2061163368 cites W2163421864 @default.
• W2061163368 cites W2178052495 @default.
• W2061163368 cites W2330076294 @default.
• W2061163368 cites W2330353674 @default.
• W2061163368 cites W2333292271 @default.
• W2061163368 cites W4236791366 @default.
• W2061163368 cites W4249862524 @default.
• W2061163368 cites W954261861 @default.
• W2061163368 doi "https://doi.org/10.1053/gast.2001.24843" @default.
• W2061163368 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11375951" @default.
• W2061163368 hasPublicationYear "2001" @default.
• W2061163368 type Work @default.
• W2061163368 sameAs 2061163368 @default.
• W2061163368 citedByCount "53" @default.
• W2061163368 countsByYear W20611633682012 @default.
• W2061163368 countsByYear W20611633682013 @default.
• W2061163368 countsByYear W20611633682014 @default.
• W2061163368 countsByYear W20611633682015 @default.
• W2061163368 countsByYear W20611633682016 @default.
• W2061163368 countsByYear W20611633682018 @default.
• W2061163368 countsByYear W20611633682019 @default.
• W2061163368 countsByYear W20611633682022 @default.
• W2061163368 crossrefType "journal-article" @default.
• W2061163368 hasAuthorship W2061163368A5005112247 @default.
• W2061163368 hasAuthorship W2061163368A5030030043 @default.
• W2061163368 hasAuthorship W2061163368A5031508697 @default.
• W2061163368 hasAuthorship W2061163368A5035049656 @default.
• W2061163368 hasAuthorship W2061163368A5036288948 @default.
• W2061163368 hasAuthorship W2061163368A5058117904 @default.
• W2061163368 hasAuthorship W2061163368A5080181876 @default.
• W2061163368 hasConcept C153911025 @default.
• W2061163368 hasConcept C190283241 @default.
• W2061163368 hasConcept C195794163 @default.
• W2061163368 hasConcept C54355233 @default.
• W2061163368 hasConcept C553184892 @default.
• W2061163368 hasConcept C55493867 @default.
• W2061163368 hasConcept C62112901 @default.
• W2061163368 hasConcept C62478195 @default.
• W2061163368 hasConcept C81885089 @default.
• W2061163368 hasConcept C86803240 @default.
• W2061163368 hasConcept C95444343 @default.
• W2061163368 hasConceptScore W2061163368C153911025 @default.
• W2061163368 hasConceptScore W2061163368C190283241 @default.
• W2061163368 hasConceptScore W2061163368C195794163 @default.
• W2061163368 hasConceptScore W2061163368C54355233 @default.

• next