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- W2061176310 abstract "Helicases and translocases are proteins that use the energy derived from ATP hydrolysis to move along or pump nucleic acid substrates. Single molecule manipulation has proved to be a powerful tool to investigate the mechanochemistry of these motors. Here we first describe the basic mechanical properties of DNA unraveled by single molecule manipulation techniques. Then we demonstrate how the knowledge of these properties has been used to design single molecule assays to address the enzymatic mechanisms of different translocases. We report on four single molecule manipulation systems addressing the mechanism of different helicases using specifically designed DNA substrates: UvrD enzyme activity detection on a stretched nicked DNA molecule, HCV NS3 helicase unwinding of a RNA hairpin under tension, the observation of RecBCD helicase/nuclease forward and backward motion, and T7 gp4 helicase mediated opening of a synthetic DNA replication fork. We then discuss experiments on two dsDNA translocases: the RuvAB motor studied on its natural substrate, the Holliday junction, and the chromosome-segregation motor FtsK, showing its unusual coupling to DNA supercoiling." @default.
- W2061176310 created "2016-06-24" @default.
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- W2061176310 date "2006-08-25" @default.
- W2061176310 modified "2023-10-18" @default.
- W2061176310 title "DNA mechanics as a tool to probe helicase and translocase activity" @default.
- W2061176310 cites W1484664508 @default.
- W2061176310 cites W1498020501 @default.
- W2061176310 cites W1503666504 @default.
- W2061176310 cites W1517376495 @default.
- W2061176310 cites W1563322259 @default.
- W2061176310 cites W1575110117 @default.
- W2061176310 cites W1817473332 @default.
- W2061176310 cites W1963485674 @default.
- W2061176310 cites W1968367762 @default.
- W2061176310 cites W1969965681 @default.
- W2061176310 cites W1970611074 @default.
- W2061176310 cites W1972069400 @default.
- W2061176310 cites W1973365755 @default.
- W2061176310 cites W1975928024 @default.
- W2061176310 cites W1976310910 @default.
- W2061176310 cites W1983655186 @default.
- W2061176310 cites W1992180542 @default.
- W2061176310 cites W1993109344 @default.
- W2061176310 cites W1993964809 @default.
- W2061176310 cites W1994141427 @default.
- W2061176310 cites W1997748760 @default.
- W2061176310 cites W2000936608 @default.
- W2061176310 cites W2001907809 @default.
- W2061176310 cites W2005149945 @default.
- W2061176310 cites W2005378404 @default.
- W2061176310 cites W2005439356 @default.
- W2061176310 cites W2008424177 @default.
- W2061176310 cites W2008848623 @default.
- W2061176310 cites W2014193559 @default.
- W2061176310 cites W2021365722 @default.
- W2061176310 cites W2036475903 @default.
- W2061176310 cites W2041008570 @default.
- W2061176310 cites W2042129050 @default.
- W2061176310 cites W2043643905 @default.
- W2061176310 cites W2051959261 @default.
- W2061176310 cites W2054281275 @default.
- W2061176310 cites W2063402277 @default.
- W2061176310 cites W2065013877 @default.
- W2061176310 cites W2067206089 @default.
- W2061176310 cites W2073277085 @default.
- W2061176310 cites W2074636264 @default.
- W2061176310 cites W2080604685 @default.
- W2061176310 cites W2082753131 @default.
- W2061176310 cites W2087028051 @default.
- W2061176310 cites W2088561792 @default.
- W2061176310 cites W2089433236 @default.
- W2061176310 cites W2092943444 @default.
- W2061176310 cites W2097293881 @default.
- W2061176310 cites W2097560797 @default.
- W2061176310 cites W2110691897 @default.
- W2061176310 cites W2110943589 @default.
- W2061176310 cites W2122708630 @default.
- W2061176310 cites W2123893936 @default.
- W2061176310 cites W2129533327 @default.
- W2061176310 cites W2131345241 @default.
- W2061176310 cites W2132315489 @default.
- W2061176310 cites W2132533277 @default.
- W2061176310 cites W2133290100 @default.
- W2061176310 cites W2135625588 @default.
- W2061176310 cites W2136290525 @default.
- W2061176310 cites W2139576948 @default.
- W2061176310 cites W2141634848 @default.
- W2061176310 cites W2145304961 @default.
- W2061176310 cites W2145894851 @default.
- W2061176310 cites W2147220402 @default.
- W2061176310 cites W2150022567 @default.
- W2061176310 cites W2150335921 @default.
- W2061176310 cites W2158661016 @default.
- W2061176310 cites W2163548075 @default.
- W2061176310 cites W2166372815 @default.
- W2061176310 cites W2167150894 @default.
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- W2061176310 doi "https://doi.org/10.1093/nar/gkl451" @default.
- W2061176310 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1616950" @default.
- W2061176310 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16935884" @default.
- W2061176310 hasPublicationYear "2006" @default.
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