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- W2061230470 abstract "The purpose of investigation was to determine the time of cell population doubling in monolayer cultures in account with the statistical optimum existing during calculation of homogeneous and independent particles in a counting chamber. The use of the statistical optimum enables operation with the mean numbers of cells per section of the counting chamber chosen as the conventional calculation unit, which accounts for correspondence under experimental conditions with some basic characteristics of Poisson's distribution and eliminates the influence of the error of random distribution of cells in the counting chamber on the variation of experimental data. In cultivation of HeLa and DAPT cells in Carrels's flasks with a diameter of 5 cm at 36°C on medium 199 with an addition of 20% bovine serum (original concentration 105 ml, suspension volume 5 ml, change of medium once in every five days) the time of HeLa cell population doubling, calculated by the analytical and graphic methods, was equal to 79.4 and 80.2 respectively, and that for DAPT cells to 144.4 and 144.5 h. The proposed method for determination of the time of population doubling may be of importance for control of the standards of media and sera used for cultivation and for investigation of the influence of various chemical and biological factors on the speed of cell growth in vitro." @default.
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- W2061230470 title "[Determination of the time for population doubling in monolayer cultures]." @default.
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