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• W2061301498 abstract "Abstract Background Several mechanisms operate during mitosis to ensure accurate chromosome segregation. However, during tumour evolution these mechanisms go awry resulting in chromosome instability. While several lines of evidence suggest that mutations in adenomatous polyposis coli ( APC ) may promote chromosome instability, at least in colon cancer, the underlying mechanisms remain unclear. Here, we turn our attention to GSK-3 – a protein kinase, which in concert with APC, targets β-catenin for proteolysis – and ask whether GSK-3 is required for accurate chromosome segregation. Results To probe the role of GSK-3 in mitosis, we inhibited GSK-3 kinase activity in cells using a panel of small molecule inhibitors, including SB-415286, AR-A014418, 1-Azakenpaullone and CHIR99021. Analysis of synchronised HeLa cells shows that GSK-3 inhibitors do not prevent G1/S progression or cell division. They do, however, significantly delay mitotic exit, largely because inhibitor-treated cells have difficulty aligning all their chromosomes. Although bipolar spindles form and the majority of chromosomes biorient, one or more chromosomes often remain mono-oriented near the spindle poles. Despite a prolonged mitotic delay, anaphase frequently initiates without the last chromosome aligning, resulting in chromosome non-disjunction. To rule out the possibility of off-target effects, we also used RNA interference to selectively repress GSK-3β. Cells deficient for GSK-3β exhibit a similar chromosome alignment defect, with chromosomes clustered near the spindle poles. GSK-3β repression also results in cells accumulating micronuclei, a hallmark of chromosome missegregation. Conclusion Thus, not only do our observations indicate a role for GSK-3 in accurate chromosome segregation, but they also raise the possibility that, if used as therapeutic agents, GSK-3 inhibitors may induce unwanted side effects by inducing chromosome instability." @default.
• W2061301498 created "2016-06-24" @default.
• W2061301498 creator A5012213186 @default.
• W2061301498 creator A5015216205 @default.
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• W2061301498 date "2007-08-14" @default.
• W2061301498 modified "2023-09-26" @default.
• W2061301498 title "GSK-3 inhibitors induce chromosome instability" @default.
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• W2061301498 cites W1927775801 @default.
• W2061301498 cites W1969646917 @default.
• W2061301498 cites W1972343960 @default.
• W2061301498 cites W1975079427 @default.
• W2061301498 cites W1979136506 @default.
• W2061301498 cites W1979993283 @default.
• W2061301498 cites W1981073960 @default.
• W2061301498 cites W1982958012 @default.
• W2061301498 cites W1984012038 @default.
• W2061301498 cites W1989582949 @default.
• W2061301498 cites W1995377960 @default.
• W2061301498 cites W1996982933 @default.
• W2061301498 cites W1997937385 @default.
• W2061301498 cites W1998170462 @default.
• W2061301498 cites W1999316583 @default.
• W2061301498 cites W2005510835 @default.
• W2061301498 cites W2006811655 @default.
• W2061301498 cites W2016515706 @default.
• W2061301498 cites W2018711267 @default.
• W2061301498 cites W2028984447 @default.
• W2061301498 cites W2033580919 @default.
• W2061301498 cites W2037607661 @default.
• W2061301498 cites W2042231416 @default.
• W2061301498 cites W2051788298 @default.
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• W2061301498 cites W2062934426 @default.
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• W2061301498 cites W2064913654 @default.
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• W2061301498 cites W2081550264 @default.
• W2061301498 cites W2083308747 @default.
• W2061301498 cites W2084567406 @default.
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• W2061301498 cites W2093155812 @default.
• W2061301498 cites W2094533427 @default.
• W2061301498 cites W2098250898 @default.
• W2061301498 cites W2101610624 @default.
• W2061301498 cites W2103292932 @default.
• W2061301498 cites W2105337907 @default.
• W2061301498 cites W2105717889 @default.
• W2061301498 cites W2108670406 @default.
• W2061301498 cites W2108688550 @default.
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• W2061301498 cites W2161226668 @default.
• W2061301498 cites W2161772474 @default.
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• W2061301498 cites W2167290154 @default.
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• W2061301498 doi "https://doi.org/10.1186/1471-2121-8-34" @default.
• W2061301498 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1976608" @default.
• W2061301498 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17697341" @default.
• W2061301498 hasPublicationYear "2007" @default.
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