FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W206131571> ?p ?o ?g. }

• W206131571 abstract "With the ever increasing number of completely sequenced prokaryotic genomes and the subsequent use of functional genomics tools, e.g. DNA microarray and proteomics, computational data analysis and the integration of microbial and molecular data is inevitable. This thesis describes the computational analyses on (hyper)thermophilic archaeal and bacterial genomes with a particular emphasis on carbohydrate metabolic pathways and their regulation. These analyses were integrated with wet-lab functional genomics data and results from classical molecular biology and microbial physiology experiments. The research was conducted on the archaea Sulfolobus solfataricus, Pyrococcus furiosus, T. kodakaraensis and the hydrogen producing bacterium Caldicellulosiruptor saccharolyticus. The reconstruction of the central carbohydrate metabolism in the thermo-acidophile S. solfataricus was carried out by a combination of genome sequence, whole transcriptome and proteome analyses. Only slight differences in the mRNA and the protein expression levels were shown when S. solfataricus was grown on peptides vs. glucose. However, the breakdown of D-arabinose vs. D-glucose revealed a complete novel pathway in the domain of Archaea. Similar catabolic pathways were identified in other prokaryotes and therefore a comprehensive genomic reconstruction was carried out on the pentose utilizing pathways in Archaea and, additionally, the results were compared to Bacteria and Eukarya. A computational promoter analysis of the glycolytic genes in the anaerobic species of the order Thermococcales (P. furiosus and T. kodakaraensis) indicated a clear cis-regulatory element that putatively controls all the genes of the glucose and starch degrading pathways. A comparative genomic analysis of the hyperthermophilic Thermococcales species led to the discovery of a putative transcriptional regulator that is probably involved in regulation of the entire regulon. The complete genome sequence of the extremely thermophilic Caldicellulosiruptor saccharolyticus revealed a circular genome of 2,970,275 base pairs that encodes 2679 putative proteins. The central carbohydrate pathways of C. saccharolyticus were studied in detail and the pathways for producing biohydrogen from plant cell wall material were unraveled. Subsequently, a whole transcriptome analysis of C. saccharolyticus grown on different monosaccharides showed a tight transcriptional regulation of these pathways, without glucose-based catabolite repression. C. saccharolyticus is therefore a good candidate to produce molecular hydrogen from biomass feedstock. The new insights into how prokaryotic genomes, genes and their encoded proteins function, as described in this thesis, can be applied on hyperthermophilic proteins and strains for use in and improvement of industrial processes." @default.
• W206131571 created "2016-06-24" @default.
• W206131571 creator A5008299051 @default.
• W206131571 date "2008-01-01" @default.
• W206131571 modified "2023-09-22" @default.
• W206131571 title "Computational genomics of hyperthermophiles" @default.
• W206131571 cites W1010115582 @default.
• W206131571 cites W130101901 @default.
• W206131571 cites W1480396224 @default.
• W206131571 cites W1493290107 @default.
• W206131571 cites W1501168178 @default.
• W206131571 cites W151023673 @default.
• W206131571 cites W1519987066 @default.
• W206131571 cites W1525965756 @default.
• W206131571 cites W1527056107 @default.
• W206131571 cites W1529201898 @default.
• W206131571 cites W1531341650 @default.
• W206131571 cites W1532217983 @default.
• W206131571 cites W1535830149 @default.
• W206131571 cites W1558365920 @default.
• W206131571 cites W1568513966 @default.
• W206131571 cites W1572621101 @default.
• W206131571 cites W1582358014 @default.
• W206131571 cites W1583586808 @default.
• W206131571 cites W1584653951 @default.
• W206131571 cites W1592870802 @default.
• W206131571 cites W1596543668 @default.
• W206131571 cites W1597518289 @default.
• W206131571 cites W1601289231 @default.
• W206131571 cites W1604280659 @default.
• W206131571 cites W1608218663 @default.
• W206131571 cites W1627698625 @default.
• W206131571 cites W1657410952 @default.
• W206131571 cites W1658221551 @default.
• W206131571 cites W1669459870 @default.
• W206131571 cites W1712239258 @default.
• W206131571 cites W175167995 @default.
• W206131571 cites W1771671623 @default.
• W206131571 cites W1850524686 @default.
• W206131571 cites W1856872680 @default.
• W206131571 cites W1865283356 @default.
• W206131571 cites W1889292431 @default.
• W206131571 cites W1892745379 @default.
• W206131571 cites W1935374737 @default.
• W206131571 cites W1936606969 @default.
• W206131571 cites W1947762817 @default.
• W206131571 cites W1950579636 @default.
• W206131571 cites W1964520413 @default.
• W206131571 cites W1965129150 @default.
• W206131571 cites W1967072453 @default.
• W206131571 cites W1970070209 @default.
• W206131571 cites W1970258666 @default.
• W206131571 cites W1970329923 @default.
• W206131571 cites W1970526463 @default.
• W206131571 cites W1971277373 @default.
• W206131571 cites W1971403296 @default.
• W206131571 cites W1971405046 @default.
• W206131571 cites W1972785876 @default.
• W206131571 cites W1973148665 @default.
• W206131571 cites W1973152779 @default.
• W206131571 cites W1973949920 @default.
• W206131571 cites W1975113414 @default.
• W206131571 cites W1975224955 @default.
• W206131571 cites W1977016356 @default.
• W206131571 cites W1978173716 @default.
• W206131571 cites W1978486653 @default.
• W206131571 cites W1979510496 @default.
• W206131571 cites W1979558568 @default.
• W206131571 cites W1980484671 @default.
• W206131571 cites W1980757676 @default.
• W206131571 cites W1980776051 @default.
• W206131571 cites W1981022189 @default.
• W206131571 cites W1982997474 @default.
• W206131571 cites W1983447033 @default.
• W206131571 cites W1984345785 @default.
• W206131571 cites W1984890497 @default.
• W206131571 cites W1985092735 @default.
• W206131571 cites W1986010958 @default.
• W206131571 cites W1987110404 @default.
• W206131571 cites W1987251407 @default.
• W206131571 cites W1988015925 @default.
• W206131571 cites W1990152809 @default.
• W206131571 cites W1992837433 @default.
• W206131571 cites W1994277771 @default.
• W206131571 cites W1995557066 @default.
• W206131571 cites W1995740239 @default.
• W206131571 cites W1997146378 @default.
• W206131571 cites W1998026171 @default.
• W206131571 cites W1999269740 @default.
• W206131571 cites W2000266493 @default.
• W206131571 cites W2000341955 @default.
• W206131571 cites W2002810210 @default.
• W206131571 cites W2004902488 @default.
• W206131571 cites W2005824211 @default.
• W206131571 cites W2006405683 @default.
• W206131571 cites W2007298287 @default.
• W206131571 cites W2008840623 @default.
• W206131571 cites W2009689313 @default.
• W206131571 cites W2010523840 @default.
• W206131571 cites W2011177499 @default.

• next