Matches in SemOpenAlex for { <https://semopenalex.org/work/W2061346228> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2061346228 endingPage "304" @default.
- W2061346228 startingPage "289" @default.
- W2061346228 abstract "Purified cellular retinol-binding protein (cRBP) and cellular retinoic acid-binding protein (cRABP) mediated the transfer of their respective 3H-ligands to nuclei and microsomes of retinol-sufficient rat testes interstitial cells in a saturable and reversible manner. Crossover experiments utilizing [3H]retinol·cRBP/retinoic acid·cRABP and [3H]retinoic acid·cRABP/retinol·cRBP did not result in dissociation of the labeled ligand from either the nuclear or microsomal fraction. Further, binding of free [3H]retinol or [3H]retinoic acid was not saturable nor was binding detectably reduced in the presence of unlabeled retinoids. When nuclei and microsomes were prepared from retinol-deficient tissues, cRBP-mediated binding of [3H]retinol was also saturable and reversible. However, total binding was significantly increased in both cell fractions. Similarly, cRABP-mediated binding of [3H]retinoic acid was significantly higher in retinol-deficient nuclei and microsomes. Extraction of cRBP- or cRABP-mediated nuclear-bound 3H-retinoids with NaCl indicated that 50% of the retinol and retinoic acid were removed with 0.14 M NaCl. Retinol binding in nuclear subfractions including the 0.14 M NaCl fraction was pronasesensitive, RNase and DNase insensitive; retinoic acid was pronase- and RNase-sensitive, and DNase-insensitive. Nuclear binding of free retinoids was <15% sensitive to NaCl extraction with the major portion being retained in the nuclear membrane. These data indicate that nuclear and microsomal binding of retinoic acid as well as retinol is mediated by its transport protein. Further, microsomes as well as nuclei are capable of specific binding of retinol and retinoic acid when binding is mediated by cRBP and cRABP, respectively. Retinoid binding in nuclei appears to form a complex which is predominantly protein, suggesting that retinoids do not, upon initial binding, interact directly with DNA." @default.
- W2061346228 created "2016-06-24" @default.
- W2061346228 creator A5016794828 @default.
- W2061346228 creator A5016932481 @default.
- W2061346228 creator A5068434614 @default.
- W2061346228 date "1984-03-01" @default.
- W2061346228 modified "2023-10-16" @default.
- W2061346228 title "Retinoid binding to nuclei and microsomes of rat testes interstitial cells: I — Mediation of retinoid binding by cellular retinoid-binding proteins" @default.
- W2061346228 cites W1491275120 @default.
- W2061346228 cites W1550714542 @default.
- W2061346228 cites W1576645744 @default.
- W2061346228 cites W1596173378 @default.
- W2061346228 cites W1852165985 @default.
- W2061346228 cites W1906197854 @default.
- W2061346228 cites W1989616670 @default.
- W2061346228 cites W1990234028 @default.
- W2061346228 cites W2000893580 @default.
- W2061346228 cites W2009201617 @default.
- W2061346228 cites W2019305721 @default.
- W2061346228 cites W2024184610 @default.
- W2061346228 cites W2064803733 @default.
- W2061346228 cites W2085807648 @default.
- W2061346228 cites W2095674575 @default.
- W2061346228 cites W2166456239 @default.
- W2061346228 cites W2187027444 @default.
- W2061346228 cites W2290414686 @default.
- W2061346228 cites W2334297974 @default.
- W2061346228 cites W2338308189 @default.
- W2061346228 doi "https://doi.org/10.1016/s0271-5317(84)80014-7" @default.
- W2061346228 hasPublicationYear "1984" @default.
- W2061346228 type Work @default.
- W2061346228 sameAs 2061346228 @default.
- W2061346228 citedByCount "17" @default.
- W2061346228 crossrefType "journal-article" @default.
- W2061346228 hasAuthorship W2061346228A5016794828 @default.
- W2061346228 hasAuthorship W2061346228A5016932481 @default.
- W2061346228 hasAuthorship W2061346228A5068434614 @default.
- W2061346228 hasConcept C104317684 @default.
- W2061346228 hasConcept C181199279 @default.
- W2061346228 hasConcept C185592680 @default.
- W2061346228 hasConcept C2775954498 @default.
- W2061346228 hasConcept C2776940978 @default.
- W2061346228 hasConcept C2779573558 @default.
- W2061346228 hasConcept C2779963572 @default.
- W2061346228 hasConcept C2781121885 @default.
- W2061346228 hasConcept C553089730 @default.
- W2061346228 hasConcept C55493867 @default.
- W2061346228 hasConcept C86803240 @default.
- W2061346228 hasConcept C87644729 @default.
- W2061346228 hasConcept C97749175 @default.
- W2061346228 hasConceptScore W2061346228C104317684 @default.
- W2061346228 hasConceptScore W2061346228C181199279 @default.
- W2061346228 hasConceptScore W2061346228C185592680 @default.
- W2061346228 hasConceptScore W2061346228C2775954498 @default.
- W2061346228 hasConceptScore W2061346228C2776940978 @default.
- W2061346228 hasConceptScore W2061346228C2779573558 @default.
- W2061346228 hasConceptScore W2061346228C2779963572 @default.
- W2061346228 hasConceptScore W2061346228C2781121885 @default.
- W2061346228 hasConceptScore W2061346228C553089730 @default.
- W2061346228 hasConceptScore W2061346228C55493867 @default.
- W2061346228 hasConceptScore W2061346228C86803240 @default.
- W2061346228 hasConceptScore W2061346228C87644729 @default.
- W2061346228 hasConceptScore W2061346228C97749175 @default.
- W2061346228 hasIssue "2" @default.
- W2061346228 hasLocation W20613462281 @default.
- W2061346228 hasOpenAccess W2061346228 @default.
- W2061346228 hasPrimaryLocation W20613462281 @default.
- W2061346228 hasRelatedWork W169185698 @default.
- W2061346228 hasRelatedWork W1989549684 @default.
- W2061346228 hasRelatedWork W2025902228 @default.
- W2061346228 hasRelatedWork W2036650770 @default.
- W2061346228 hasRelatedWork W2048613378 @default.
- W2061346228 hasRelatedWork W2050815586 @default.
- W2061346228 hasRelatedWork W2055609582 @default.
- W2061346228 hasRelatedWork W2094271151 @default.
- W2061346228 hasRelatedWork W2110376098 @default.
- W2061346228 hasRelatedWork W2118166718 @default.
- W2061346228 hasVolume "4" @default.
- W2061346228 isParatext "false" @default.
- W2061346228 isRetracted "false" @default.
- W2061346228 magId "2061346228" @default.
- W2061346228 workType "article" @default.