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• W2061487060 abstract "During the construction of the Messing pUC plasmid series, the rop(rom) gene of pBR322 which mediates the activity of RNAI was deleted. This has resulted in an elevated copy number for the pUC plasmids which makes the expression of β-galactosidase activity constitutive in a host containing the Iqtss lac repressor. We describe the construction of a new series of vectors which retain the pUC multiple cloning site (MCS) but in which copy number control has been recovered. In addition, the lacα/lac promoter expression region has been inserted into a HpaI cassette. This facilitates the movement of recombinant DNA clones within the MCS. It also increases the complementation activity of the lacα peptide by an order of magnitude, allowing selection of recombinants by their Lac− phenotype on MacConkey agar." @default.
• W2061487060 created "2016-06-24" @default.
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• W2061487060 date "1986-05-01" @default.
• W2061487060 modified "2023-09-27" @default.
• W2061487060 title "pHG165: A pBR322 copy number derivative of pUC8 for cloning and expression" @default.
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• W2061487060 doi "https://doi.org/10.1016/0147-619x(86)90035-1" @default.
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