FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2061528612> ?p ?o ?g. }

• W2061528612 endingPage "75" @default.
• W2061528612 startingPage "69" @default.
• W2061528612 abstract "We have previously reported that 1alpha,25-dihydroxyvitamin D3 [1alpha,25(OH)2D3] accumulates in the dialysis fluid of uremic patients treated by continuous ambulatory peritoneal dialysis (CAPD). It has been reported that this metabolite regulates the production of cytokines by monocytes/macrophages. Since tumor necrosis factor-alpha (TNF-alpha) initiates an inflammatory cascade during peritonitis, the aim of the present study was to investigate the effect of 1alpha, 25(OH)2D3 on the production of TNF-alpha by human peritoneal macrophages (HPMs).HPMs were obtained from patients on CAPD. Cells were incubated with various concentrations of 1alpha, 25(OH)2D3, 1alpha,24(S) dihydroxyvitamin D2 [1alpha,24(S)(OH)2D2] or 25-hydroxyvitamin D3 (25-OH-D3) for 16 hours. This was followed by lipopolysaccharide (LPS; 1 microg/mL) incubation for 2.5 to 6 hours. TNF-alpha protein production was determined by enzyme-linked immunosorbent assay. TNF-alpha mRNA was assayed by the reverse transcriptase-polymerase chain reaction procedure, using internal synthetic mRNA standards for quantitative results.Incubation of HPMs with 1alpha,25(OH)2D3 prior to stimulation with LPS dose dependently inhibited the expression of TNF-alpha on both mRNA and protein levels. Similar results were obtained with the less calcemic vitamin D2 analogue 1alpha,24(S)(OH)2D2. Incubation of HPMs with 25-OH-D3 also revealed a down-regulation of TNF-alpha expression. Since this down-regulatory effect was blocked by ketoconazole, it is likely that this effect was caused by the conversion of 25-OH-D3 into 1alpha,25(OH)2D3 by HPMs.1alpha,25(OH)2D3 has a potent inhibitory effect on the production of TNF-alpha by LPS-activated HPMs. We hypothesize that 1alpha, 25(OH)2D3 may constitute a regulatory mechanism that, by controlling the intensity of the inflammatory response of the peritoneum, will moderate tissue damage during peritonitis." @default.
• W2061528612 created "2016-06-24" @default.
• W2061528612 creator A5001152646 @default.
• W2061528612 creator A5040234663 @default.
• W2061528612 creator A5047803427 @default.
• W2061528612 creator A5086616361 @default.
• W2061528612 date "2001-01-01" @default.
• W2061528612 modified "2023-09-27" @default.
• W2061528612 title "Regulation of TNF-α by 1α,25-dihydroxyvitamin D3 in human macrophages from CAPD patients" @default.
• W2061528612 cites W1483907253 @default.
• W2061528612 cites W1965853172 @default.
• W2061528612 cites W1965860042 @default.
• W2061528612 cites W1972457463 @default.
• W2061528612 cites W2002209097 @default.
• W2061528612 cites W2005167663 @default.
• W2061528612 cites W2007303522 @default.
• W2061528612 cites W2011232539 @default.
• W2061528612 cites W2021409342 @default.
• W2061528612 cites W2026342001 @default.
• W2061528612 cites W2027480555 @default.
• W2061528612 cites W2028460544 @default.
• W2061528612 cites W2036088433 @default.
• W2061528612 cites W2036616769 @default.
• W2061528612 cites W2046214413 @default.
• W2061528612 cites W2048325240 @default.
• W2061528612 cites W2054692125 @default.
• W2061528612 cites W2058504391 @default.
• W2061528612 cites W2058782131 @default.
• W2061528612 cites W2065897358 @default.
• W2061528612 cites W2083373216 @default.
• W2061528612 cites W2084353045 @default.
• W2061528612 cites W2089461315 @default.
• W2061528612 cites W2095175490 @default.
• W2061528612 cites W2109168421 @default.
• W2061528612 cites W2125489531 @default.
• W2061528612 cites W2141801958 @default.
• W2061528612 cites W2142273705 @default.
• W2061528612 cites W2145446674 @default.
• W2061528612 cites W2167999248 @default.
• W2061528612 cites W2337802237 @default.
• W2061528612 doi "https://doi.org/10.1046/j.1523-1755.2001.00467.x" @default.
• W2061528612 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11135059" @default.
• W2061528612 hasPublicationYear "2001" @default.
• W2061528612 type Work @default.
• W2061528612 sameAs 2061528612 @default.
• W2061528612 citedByCount "57" @default.
• W2061528612 countsByYear W20615286122012 @default.
• W2061528612 countsByYear W20615286122013 @default.
• W2061528612 countsByYear W20615286122014 @default.
• W2061528612 countsByYear W20615286122015 @default.
• W2061528612 countsByYear W20615286122016 @default.
• W2061528612 countsByYear W20615286122017 @default.
• W2061528612 countsByYear W20615286122018 @default.
• W2061528612 countsByYear W20615286122021 @default.
• W2061528612 countsByYear W20615286122022 @default.
• W2061528612 countsByYear W20615286122023 @default.
• W2061528612 crossrefType "journal-article" @default.
• W2061528612 hasAuthorship W2061528612A5001152646 @default.
• W2061528612 hasAuthorship W2061528612A5040234663 @default.
• W2061528612 hasAuthorship W2061528612A5047803427 @default.
• W2061528612 hasAuthorship W2061528612A5086616361 @default.
• W2061528612 hasBestOaLocation W20615286121 @default.
• W2061528612 hasConcept C124490489 @default.
• W2061528612 hasConcept C126322002 @default.
• W2061528612 hasConcept C134018914 @default.
• W2061528612 hasConcept C153639570 @default.
• W2061528612 hasConcept C179639408 @default.
• W2061528612 hasConcept C17991360 @default.
• W2061528612 hasConcept C25642318 @default.
• W2061528612 hasConcept C2776079296 @default.
• W2061528612 hasConcept C2776940978 @default.
• W2061528612 hasConcept C2778754761 @default.
• W2061528612 hasConcept C2779056158 @default.
• W2061528612 hasConcept C2909406889 @default.
• W2061528612 hasConcept C55493867 @default.
• W2061528612 hasConcept C71924100 @default.
• W2061528612 hasConcept C86803240 @default.
• W2061528612 hasConceptScore W2061528612C124490489 @default.
• W2061528612 hasConceptScore W2061528612C126322002 @default.
• W2061528612 hasConceptScore W2061528612C134018914 @default.
• W2061528612 hasConceptScore W2061528612C153639570 @default.
• W2061528612 hasConceptScore W2061528612C179639408 @default.
• W2061528612 hasConceptScore W2061528612C17991360 @default.
• W2061528612 hasConceptScore W2061528612C25642318 @default.
• W2061528612 hasConceptScore W2061528612C2776079296 @default.
• W2061528612 hasConceptScore W2061528612C2776940978 @default.
• W2061528612 hasConceptScore W2061528612C2778754761 @default.
• W2061528612 hasConceptScore W2061528612C2779056158 @default.
• W2061528612 hasConceptScore W2061528612C2909406889 @default.
• W2061528612 hasConceptScore W2061528612C55493867 @default.
• W2061528612 hasConceptScore W2061528612C71924100 @default.
• W2061528612 hasConceptScore W2061528612C86803240 @default.
• W2061528612 hasIssue "1" @default.
• W2061528612 hasLocation W20615286121 @default.
• W2061528612 hasLocation W20615286122 @default.
• W2061528612 hasOpenAccess W2061528612 @default.
• W2061528612 hasPrimaryLocation W20615286121 @default.
• W2061528612 hasRelatedWork W2003942877 @default.

• next