Matches in SemOpenAlex for { <https://semopenalex.org/work/W2061588045> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2061588045 endingPage "193" @default.
- W2061588045 startingPage "190" @default.
- W2061588045 abstract "The majority of the world's human population experiences a decline of lactase gene expression during maturation, so-called lactase nonpersistence. Thus, adults with lactase nonpersistence are susceptible to developing symptoms of lactose intolerance. By contrast, lactase persistence is an autosomal dominant heritable condition that results in a high level of lactase gene expression throughout adulthood and sustained lactose tolerance. Lactase persistence has recently been correlated with a single nucleotide genetic variant (a C --> T mutation) located 13,910 bases upstream from the lactase structural gene. We aimed to develop a restriction fragment length polymorphism (RFLP) method of detecting the C/T variants as a means of identifying individuals genetically inclined toward lactase persistence or nonpersistence. Genomic DNA in a 210-bp region surrounding the -13,910-bp variant site was PCR amplified with unique primers designed to avoid or mutate adjacent restriction sites. The amplified DNA was digested with a restriction enzyme, CviJI, that recognizes the base pair sequence generated by the lactase nonpersistence variant. Restriction digest gel analysis yielded DNA fragments of the expected diagnostic molecular weight sizes for individuals that were homozygote or heterozygote for the lactase persistence and nonpersistence variants. The genotypes predicted by the RFLP-based method were confirmed by DNA sequence analysis. The RFLP-based method provides a quick and noninvasive means of molecular detection of the presence or absence of the lactase persistence variant." @default.
- W2061588045 created "2016-06-24" @default.
- W2061588045 creator A5004295821 @default.
- W2061588045 creator A5013929476 @default.
- W2061588045 date "2004-06-01" @default.
- W2061588045 modified "2023-09-30" @default.
- W2061588045 title "PCR-RFLP Genotyping Assay for a Lactase Persistence Polymorphism Upstream of the Lactase-Phlorizin Hydrolase Gene" @default.
- W2061588045 cites W2001722477 @default.
- W2061588045 cites W2012345358 @default.
- W2061588045 cites W2019368267 @default.
- W2061588045 cites W2067991539 @default.
- W2061588045 cites W2135841997 @default.
- W2061588045 cites W220133576 @default.
- W2061588045 cites W4250654678 @default.
- W2061588045 doi "https://doi.org/10.1089/gte.2004.8.190" @default.
- W2061588045 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15345119" @default.
- W2061588045 hasPublicationYear "2004" @default.
- W2061588045 type Work @default.
- W2061588045 sameAs 2061588045 @default.
- W2061588045 citedByCount "25" @default.
- W2061588045 countsByYear W20615880452012 @default.
- W2061588045 countsByYear W20615880452013 @default.
- W2061588045 countsByYear W20615880452014 @default.
- W2061588045 countsByYear W20615880452015 @default.
- W2061588045 countsByYear W20615880452016 @default.
- W2061588045 countsByYear W20615880452023 @default.
- W2061588045 crossrefType "journal-article" @default.
- W2061588045 hasAuthorship W2061588045A5004295821 @default.
- W2061588045 hasAuthorship W2061588045A5013929476 @default.
- W2061588045 hasConcept C104317684 @default.
- W2061588045 hasConcept C128319531 @default.
- W2061588045 hasConcept C135763542 @default.
- W2061588045 hasConcept C144024400 @default.
- W2061588045 hasConcept C149923435 @default.
- W2061588045 hasConcept C153911025 @default.
- W2061588045 hasConcept C168007829 @default.
- W2061588045 hasConcept C2777432071 @default.
- W2061588045 hasConcept C2778724459 @default.
- W2061588045 hasConcept C2778974006 @default.
- W2061588045 hasConcept C2908647359 @default.
- W2061588045 hasConcept C31467283 @default.
- W2061588045 hasConcept C54355233 @default.
- W2061588045 hasConcept C55493867 @default.
- W2061588045 hasConcept C86803240 @default.
- W2061588045 hasConceptScore W2061588045C104317684 @default.
- W2061588045 hasConceptScore W2061588045C128319531 @default.
- W2061588045 hasConceptScore W2061588045C135763542 @default.
- W2061588045 hasConceptScore W2061588045C144024400 @default.
- W2061588045 hasConceptScore W2061588045C149923435 @default.
- W2061588045 hasConceptScore W2061588045C153911025 @default.
- W2061588045 hasConceptScore W2061588045C168007829 @default.
- W2061588045 hasConceptScore W2061588045C2777432071 @default.
- W2061588045 hasConceptScore W2061588045C2778724459 @default.
- W2061588045 hasConceptScore W2061588045C2778974006 @default.
- W2061588045 hasConceptScore W2061588045C2908647359 @default.
- W2061588045 hasConceptScore W2061588045C31467283 @default.
- W2061588045 hasConceptScore W2061588045C54355233 @default.
- W2061588045 hasConceptScore W2061588045C55493867 @default.
- W2061588045 hasConceptScore W2061588045C86803240 @default.
- W2061588045 hasIssue "2" @default.
- W2061588045 hasLocation W20615880451 @default.
- W2061588045 hasLocation W20615880452 @default.
- W2061588045 hasOpenAccess W2061588045 @default.
- W2061588045 hasPrimaryLocation W20615880451 @default.
- W2061588045 hasRelatedWork W1579334360 @default.
- W2061588045 hasRelatedWork W2039709676 @default.
- W2061588045 hasRelatedWork W2068362112 @default.
- W2061588045 hasRelatedWork W2097518733 @default.
- W2061588045 hasRelatedWork W2106455172 @default.
- W2061588045 hasRelatedWork W2114641189 @default.
- W2061588045 hasRelatedWork W2129166058 @default.
- W2061588045 hasRelatedWork W2158510364 @default.
- W2061588045 hasRelatedWork W2417746725 @default.
- W2061588045 hasRelatedWork W3031643465 @default.
- W2061588045 hasVolume "8" @default.
- W2061588045 isParatext "false" @default.
- W2061588045 isRetracted "false" @default.
- W2061588045 magId "2061588045" @default.
- W2061588045 workType "article" @default.