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• W2061618744 abstract "The aim of this work is to evaluate the gonadotropin and growth factor effects in vitro on steroidal response in human granulosa luteal cells from polycystic ovaries compared with normal granulosa luteal cells in humans. The granulosa cells from polycystic (polycystic ovarian granulosa cells, POGC) and normo-ovulating women (normal cells, NC) were collected in the preovulatory phase after oocyte retrieval during the GIFT program. The cells were cultured serum-free for 24, 48 and 96 h. Estradiol and progesterone production was determined with or without HCG (1–200 ng/ml), FSH (10–300 ng/ml), insulin (1–50μ/ml) and IGF I (1–50 ng/ml) addition. All treatments significantly induced a 2–3 fold estradiol increase at the 48-h and 96-h time points in POGC. The progesterone production was unaffected by HCG, FSH, insulin and IGF I addition, respectively, in POGC, whereas the NC were responsive at the 48-h and 96-h time points. FSH did not stimulate progesterone production in granulosa cells either from polycystic or normovulating subjects. Our findings indicate that POGC are hypersensitive to all substances in terms of estradiol production, whereas they show a reduced capacity of progesterone production with some treatments." @default.
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• W2061618744 date "1994-12-01" @default.
• W2061618744 modified "2023-10-01" @default.
• W2061618744 title "Effect of gonadotropins, insulin and IGF I on granulosa luteal cells from polycystic ovaries" @default.
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• W2061618744 doi "https://doi.org/10.1016/0303-7207(94)90190-2" @default.
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