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- W2061623215 abstract "HD263 is a recessive temperature-sensitive mutation which maps in the promoter proximal region of the rIIB gene of the bacteriophage T4. Expression of rIIB is temperature-dependent in this mutant strain. No rIIB polypeptide is found in HD263 infections of Escherichia coli B at high temperature, and at low temperature less rIIB polypeptide is found than in equivalent wild-type infections. This accounts for the r-type plaque which HD263 forms when plated on E. coli B at any temperature, and for the inability of HD263 to grow on a lambda lysogen at high temperature. No other differences between HD263 and wild type have been found. Possible mechanisms to account for the temperature sensitivity of HD263 have been considered. Temperature-sensitive transcription has been ruled out. Degradation of HD263 rIIB polypeptide has been impossible to detect at any temperature. This suggests that the translatability of HD263 rIIB mRNA is temperature-dependent. A revertant of HD263 which is no longer temperature-sensitive but which produces limited quantities of rIIB polypeptide is discussed. The properties of this revertant are consistent with a translational model for the temperature sensitivity of HD263." @default.
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- W2061623215 date "1976-05-01" @default.
- W2061623215 modified "2023-10-16" @default.
- W2061623215 title "A mutation that confers temperature sensitivity on the translation of rIIB in bacteriophage T4" @default.
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- W2061623215 doi "https://doi.org/10.1016/0022-2836(76)90221-7" @default.
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