FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2061635669> ?p ?o ?g. }

• W2061635669 endingPage "1248" @default.
• W2061635669 startingPage "1233" @default.
• W2061635669 abstract "Abstract The role of the proximal promoter GC‐box in regulating basal and cAMP‐dependent GTP Cyclohydrolase I gene transcription was investigated using a variety of cell lines and techniques. These studies show that the GC‐box is composed of a triad of cis ‐elements that in vitro bind specificity proteins Sp1 and Sp3 . Sp1 and Sp3 were found associated with the native proximal promoter in PC12 cells but were not recruited to the promoter during cAMP‐dependent transcription. Studies using Drosophila SL2 cells showed that Sp3 occupies two sites within the GC‐box and enhances transcription when acting alone and synergistically when combined with nuclear factor‐Y (NF‐Y) and CCAAT/Enhancer‐Binding Protein (C/EBP)β, cognate binding proteins for the adjacent cAMP response element (CRE) and CCAAT‐box cAMP response elements. In contrast, Sp1 bound only one site within the GC‐box and did not enhance transcription unless combined with NF‐Y and C/EBPβ. Studies in SL2 cells also showed that Sp1 and Sp3 do not co‐occupy the GC‐box, and accordingly Sp1 competes for Sp3 binding to repress Sp3‐dependent transcription. In PC12 cells, complete mutation of the GC‐box reduced basal but not cAMP‐dependent transcription, resulting in an overall increase in the cAMP response and demonstrating that formation of this enhanceosome does not require Sp1 or Sp3. Experiments in which the GC‐box was replaced with a Gal4 element and the promoter challenged with Gal4 fusion proteins support this conclusion and a role for Sp3 in maintaining high levels of basal transcription in PC12 cells. Equivalent amounts of Sp1 and Sp3 were found associated with the native proximal promoter in PC12 and Rat2 cells, which differ 10‐fold in basal transcription. Similar levels of methylation of CpG dinucleotides located within the GC‐box were also observed in these two cells lines. These results suggest that Sp1 and Sp3 bound to the GC‐box might help to preserve an open chromatin configuration at the proximal promoter in cells which constitutively express low levels of GTP Cyclohydrolase I." @default.
• W2061635669 created "2016-06-24" @default.
• W2061635669 creator A5054564145 @default.
• W2061635669 creator A5058183240 @default.
• W2061635669 creator A5059771285 @default.
• W2061635669 creator A5077565874 @default.
• W2061635669 date "2007-10-09" @default.
• W2061635669 modified "2023-09-29" @default.
• W2061635669 title "Specificity proteins Sp1 and Sp3 interact with the rat GTP Cyclohydrolase I proximal promoter to regulate transcription" @default.
• W2061635669 cites W1228798347 @default.
• W2061635669 cites W1533126574 @default.
• W2061635669 cites W1533717041 @default.
• W2061635669 cites W1544307004 @default.
• W2061635669 cites W1554534316 @default.
• W2061635669 cites W1580718840 @default.
• W2061635669 cites W1795977892 @default.
• W2061635669 cites W1922521877 @default.
• W2061635669 cites W1940871001 @default.
• W2061635669 cites W1967064280 @default.
• W2061635669 cites W1969599461 @default.
• W2061635669 cites W1977344247 @default.
• W2061635669 cites W1979342595 @default.
• W2061635669 cites W1980273804 @default.
• W2061635669 cites W1983803117 @default.
• W2061635669 cites W1986473194 @default.
• W2061635669 cites W1986868728 @default.
• W2061635669 cites W1989142029 @default.
• W2061635669 cites W1990189563 @default.
• W2061635669 cites W1990240568 @default.
• W2061635669 cites W1993063708 @default.
• W2061635669 cites W1993243592 @default.
• W2061635669 cites W1996173977 @default.
• W2061635669 cites W1997834806 @default.
• W2061635669 cites W1999156595 @default.
• W2061635669 cites W2001288516 @default.
• W2061635669 cites W2002000066 @default.
• W2061635669 cites W2006362317 @default.
• W2061635669 cites W2006486865 @default.
• W2061635669 cites W2008086014 @default.
• W2061635669 cites W2008698762 @default.
• W2061635669 cites W2010499718 @default.
• W2061635669 cites W2011975888 @default.
• W2061635669 cites W2012383881 @default.
• W2061635669 cites W2028729871 @default.
• W2061635669 cites W2030072196 @default.
• W2061635669 cites W2037653294 @default.
• W2061635669 cites W2038183822 @default.
• W2061635669 cites W2040257851 @default.
• W2061635669 cites W2045132122 @default.
• W2061635669 cites W2045679054 @default.
• W2061635669 cites W2048655016 @default.
• W2061635669 cites W2061274497 @default.
• W2061635669 cites W2063767041 @default.
• W2061635669 cites W2079891060 @default.
• W2061635669 cites W2081085542 @default.
• W2061635669 cites W2082012238 @default.
• W2061635669 cites W2083819673 @default.
• W2061635669 cites W2089628848 @default.
• W2061635669 cites W2091157788 @default.
• W2061635669 cites W2094105923 @default.
• W2061635669 cites W2106169455 @default.
• W2061635669 cites W2111775919 @default.
• W2061635669 cites W2128399345 @default.
• W2061635669 cites W2139122477 @default.
• W2061635669 cites W2142257447 @default.
• W2061635669 cites W2154932077 @default.
• W2061635669 cites W2164966658 @default.
• W2061635669 cites W2168805141 @default.
• W2061635669 cites W2171560459 @default.
• W2061635669 cites W2418361432 @default.
• W2061635669 cites W3119630387 @default.
• W2061635669 cites W4231928998 @default.
• W2061635669 cites W4293247451 @default.
• W2061635669 cites W70806202 @default.
• W2061635669 doi "https://doi.org/10.1111/j.1471-4159.2007.05054.x" @default.
• W2061635669 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2265209" @default.
• W2061635669 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18004997" @default.
• W2061635669 hasPublicationYear "2007" @default.
• W2061635669 type Work @default.
• W2061635669 sameAs 2061635669 @default.
• W2061635669 citedByCount "11" @default.
• W2061635669 countsByYear W20616356692012 @default.
• W2061635669 countsByYear W20616356692013 @default.
• W2061635669 countsByYear W20616356692022 @default.
• W2061635669 crossrefType "journal-article" @default.
• W2061635669 hasAuthorship W2061635669A5054564145 @default.
• W2061635669 hasAuthorship W2061635669A5058183240 @default.
• W2061635669 hasAuthorship W2061635669A5059771285 @default.
• W2061635669 hasAuthorship W2061635669A5077565874 @default.
• W2061635669 hasBestOaLocation W20616356692 @default.
• W2061635669 hasConcept C101762097 @default.
• W2061635669 hasConcept C104317684 @default.
• W2061635669 hasConcept C111936080 @default.
• W2061635669 hasConcept C132376346 @default.
• W2061635669 hasConcept C138885662 @default.
• W2061635669 hasConcept C144825837 @default.
• W2061635669 hasConcept C149240914 @default.
• W2061635669 hasConcept C150194340 @default.

• next