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- W2061635752 abstract "The rapid increase in the prevalence of chronic heart failure (CHF) worldwide underscores an urgent need to identify biomarkers for the early detection of CHF. Post-translational modifications (PTMs) are associated with many critical signaling events during disease progression and thus offer a plethora of candidate biomarkers. We have employed a top-down quantitative proteomics methodology for comprehensive assessment of PTMs in whole proteins extracted from normal and diseased tissues. We systematically analyzed 36 clinical human heart tissue samples and identified phosphorylation of cardiac troponin I (cTnI) as a candidate biomarker for CHF. The relative percentages of the total phosphorylated cTnI forms over the entire cTnI populations (%Ptotal) were 56.4 ± 3.5%, 36.9 ± 1.6%, 6.1 ± 2.4%, and 1.0 ± 0.6% for postmortem hearts with normal cardiac function (n = 7), early stage of mild hypertrophy (n = 5), severe hypertrophy/dilation (n = 4), and end-stage CHF (n = 6), respectively. In fresh transplant samples, the %Ptotal of cTnI from nonfailing donor (n = 4), and end-stage failing hearts (n = 10) were 49.5 ± 5.9% and 18.8 ± 2.9%, respectively. Top-down MS with electron capture dissociation unequivocally localized the altered phosphorylation sites to Ser22/23 and determined the order of phosphorylation/dephosphorylation. This study represents the first clinical application of top-down MS-based quantitative proteomics for biomarker discovery from tissues, highlighting the potential of PTMs as disease biomarkers." @default.
- W2061635752 created "2016-06-24" @default.
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- W2061635752 date "2011-07-28" @default.
- W2061635752 modified "2023-10-18" @default.
- W2061635752 title "Top-Down Quantitative Proteomics Identified Phosphorylation of Cardiac Troponin I as a Candidate Biomarker for Chronic Heart Failure" @default.
- W2061635752 cites W1530371703 @default.
- W2061635752 cites W1900726394 @default.
- W2061635752 cites W1964747697 @default.
- W2061635752 cites W1965920802 @default.
- W2061635752 cites W1970400205 @default.
- W2061635752 cites W1971687277 @default.
- W2061635752 cites W1977291445 @default.
- W2061635752 cites W1978063892 @default.
- W2061635752 cites W1979258878 @default.
- W2061635752 cites W1989841960 @default.
- W2061635752 cites W1994543305 @default.
- W2061635752 cites W1996780591 @default.
- W2061635752 cites W2000914251 @default.
- W2061635752 cites W2002845761 @default.
- W2061635752 cites W2004558299 @default.
- W2061635752 cites W2014578458 @default.
- W2061635752 cites W2023232013 @default.
- W2061635752 cites W2027875821 @default.
- W2061635752 cites W2032232569 @default.
- W2061635752 cites W2034703835 @default.
- W2061635752 cites W2036604104 @default.
- W2061635752 cites W2037803098 @default.
- W2061635752 cites W2039291159 @default.
- W2061635752 cites W2040518216 @default.
- W2061635752 cites W2043492633 @default.
- W2061635752 cites W2045161311 @default.
- W2061635752 cites W2045800415 @default.
- W2061635752 cites W2048091951 @default.
- W2061635752 cites W2048461578 @default.
- W2061635752 cites W2048527293 @default.
- W2061635752 cites W2052231736 @default.
- W2061635752 cites W2052553045 @default.
- W2061635752 cites W2053212170 @default.
- W2061635752 cites W2058581105 @default.
- W2061635752 cites W2067978791 @default.
- W2061635752 cites W2068337616 @default.
- W2061635752 cites W2074476658 @default.
- W2061635752 cites W2075094608 @default.
- W2061635752 cites W2079056345 @default.
- W2061635752 cites W2079498453 @default.
- W2061635752 cites W2081277484 @default.
- W2061635752 cites W2083876506 @default.
- W2061635752 cites W2084239942 @default.
- W2061635752 cites W2084766638 @default.
- W2061635752 cites W2085993892 @default.
- W2061635752 cites W2086482125 @default.
- W2061635752 cites W2087431150 @default.
- W2061635752 cites W2088114600 @default.
- W2061635752 cites W2093452526 @default.
- W2061635752 cites W2094229819 @default.
- W2061635752 cites W2094415569 @default.
- W2061635752 cites W2103948934 @default.
- W2061635752 cites W2104786760 @default.
- W2061635752 cites W2106845528 @default.
- W2061635752 cites W2108121781 @default.
- W2061635752 cites W2108842111 @default.
- W2061635752 cites W2111446613 @default.
- W2061635752 cites W2119425965 @default.
- W2061635752 cites W2121863513 @default.
- W2061635752 cites W2135349862 @default.
- W2061635752 cites W2138354938 @default.
- W2061635752 cites W2139645827 @default.
- W2061635752 cites W2140639952 @default.
- W2061635752 cites W2142119280 @default.
- W2061635752 cites W2142641639 @default.
- W2061635752 cites W2143598772 @default.
- W2061635752 cites W2148344623 @default.
- W2061635752 cites W2148532515 @default.
- W2061635752 cites W2150577572 @default.
- W2061635752 cites W2151353276 @default.
- W2061635752 cites W2161091072 @default.
- W2061635752 cites W2165440393 @default.
- W2061635752 cites W2172116519 @default.
- W2061635752 cites W21843527 @default.
- W2061635752 cites W2267906346 @default.
- W2061635752 doi "https://doi.org/10.1021/pr200258m" @default.
- W2061635752 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3170873" @default.
- W2061635752 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21751783" @default.
- W2061635752 hasPublicationYear "2011" @default.
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