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- W2061644315 abstract "We have studied the DNA ploidy and the proliferative activity in 102 patients with endometrial and cervical carcinoma, by flow cytometry. Samples were excised 1 hour after bromodeoxyuridine (BrdU, 250 mg/) e.v. infusion and fixed in 70% ethanol. Nuclear DNA content and BrdU incorporation, were simultaneously determined to obtain ploidy (DNA index) and proliferative activity (BrdU-labeling index, LI). No acute toxicity or side effects related to BrdU injection were recorded. The overall feasibility of the determinations was higher than 90% (93/102). Twenty-two out of 59 (37.2%) endometrial neoplasms and 23 out of 34 (67.6%) cervical neoplasms were aneuploid, with a median DNA-index of the aneuploid peak of 1.3 and 1.4, respectively. Overall median BrdU LIs were 4.8% and 7.2%. Proliferative activity was found to be higher in aneuploid tumors (p<.05). DNA ploidy and/or BrdU-LI were not significantly related either with the clinical stage or the histopathologic grading in either tumor type. The BrdU in vivo administration coupled with bivariate FCM for measurement is a simple method that can be performed in clinical settings to better evaluate the prognostic significance of proliferative parameters in gynecological tumors." @default.
- W2061644315 created "2016-06-24" @default.
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- W2061644315 date "1994-01-01" @default.
- W2061644315 modified "2023-09-26" @default.
- W2061644315 title "DNA-PLOIDY AND PROLIFERATIVE ACTIVITY IN UTERINE-TUMORS - AN IN-VIVO STUDY USING BROMODEOXYURIDINE AND FLOW-CYTOMETRY" @default.
- W2061644315 doi "https://doi.org/10.3892/or.1.1.65" @default.
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