Matches in SemOpenAlex for { <https://semopenalex.org/work/W2061644855> ?p ?o ?g. }
- W2061644855 abstract "Abstract A sensitive quantitation system using reverse transcription-polymerase chain reaction (RTPCR) was developed to measure the low estrogen receptor (ER) mRNA levels in Japanese eel (Anguilla japonica). Two types of cytoskeletal actins, β- and γ-actins, were distinguished in Japanese eel and used as the internal control for exact quantitation. Actin and ER primers of this study annealed to different exons, allowing for the skipping of DNase treatment. Accordingly, ER and actin RT-PCR products showed a single band and were amplified with the same efficiency during PCR. The ER mRNA amount was calculated as a relative value, normalized over actin (β and γ) mRNA. The results thus obtained by RT-PCR agreed with the results from Northern blot analysis of liver from pre-vitellogenic and hormone-treated early vitellogenic eel. Using this system, the ER mRNA levels were further measured in coelomic epithelium, pituitary, brain and ovary. In the liver, ER mRNA levels of the early vitellogenic eel increased abo..." @default.
- W2061644855 created "2016-06-24" @default.
- W2061644855 creator A5000256402 @default.
- W2061644855 creator A5013176183 @default.
- W2061644855 creator A5056100957 @default.
- W2061644855 creator A5058655890 @default.
- W2061644855 creator A5064359506 @default.
- W2061644855 creator A5067846152 @default.
- W2061644855 date "1998-12-01" @default.
- W2061644855 modified "2023-09-24" @default.
- W2061644855 title "The Establishment of a Quantitative RT-PCR Assay for Estrogen Receptor mRNA in Japanese Eel, Anguilla japonica" @default.
- W2061644855 cites W1840795163 @default.
- W2061644855 cites W1848341504 @default.
- W2061644855 cites W1909940207 @default.
- W2061644855 cites W191956550 @default.
- W2061644855 cites W1964436619 @default.
- W2061644855 cites W1968374085 @default.
- W2061644855 cites W1970516412 @default.
- W2061644855 cites W1979029637 @default.
- W2061644855 cites W1980686389 @default.
- W2061644855 cites W1981306061 @default.
- W2061644855 cites W1985421324 @default.
- W2061644855 cites W1987933233 @default.
- W2061644855 cites W1995534157 @default.
- W2061644855 cites W2017466336 @default.
- W2061644855 cites W2021737076 @default.
- W2061644855 cites W2024719488 @default.
- W2061644855 cites W2025024500 @default.
- W2061644855 cites W2038020118 @default.
- W2061644855 cites W2042758500 @default.
- W2061644855 cites W2044036378 @default.
- W2061644855 cites W2046231215 @default.
- W2061644855 cites W2059477274 @default.
- W2061644855 cites W2059592319 @default.
- W2061644855 cites W2073353512 @default.
- W2061644855 cites W2073883348 @default.
- W2061644855 cites W2090146012 @default.
- W2061644855 cites W2091727882 @default.
- W2061644855 cites W2092049238 @default.
- W2061644855 cites W2092602709 @default.
- W2061644855 cites W2114882367 @default.
- W2061644855 cites W2134812217 @default.
- W2061644855 cites W2135723391 @default.
- W2061644855 cites W2144040635 @default.
- W2061644855 cites W2161623422 @default.
- W2061644855 cites W2172256092 @default.
- W2061644855 cites W2204303744 @default.
- W2061644855 cites W3174437382 @default.
- W2061644855 cites W522192065 @default.
- W2061644855 cites W63159390 @default.
- W2061644855 doi "https://doi.org/10.2108/zsj.15.923" @default.
- W2061644855 hasPublicationYear "1998" @default.
- W2061644855 type Work @default.
- W2061644855 sameAs 2061644855 @default.
- W2061644855 citedByCount "9" @default.
- W2061644855 crossrefType "journal-article" @default.
- W2061644855 hasAuthorship W2061644855A5000256402 @default.
- W2061644855 hasAuthorship W2061644855A5013176183 @default.
- W2061644855 hasAuthorship W2061644855A5056100957 @default.
- W2061644855 hasAuthorship W2061644855A5058655890 @default.
- W2061644855 hasAuthorship W2061644855A5064359506 @default.
- W2061644855 hasAuthorship W2061644855A5067846152 @default.
- W2061644855 hasBestOaLocation W20616448551 @default.
- W2061644855 hasConcept C104317684 @default.
- W2061644855 hasConcept C105580179 @default.
- W2061644855 hasConcept C121608353 @default.
- W2061644855 hasConcept C125705527 @default.
- W2061644855 hasConcept C153911025 @default.
- W2061644855 hasConcept C196843134 @default.
- W2061644855 hasConcept C2776415932 @default.
- W2061644855 hasConcept C2776690073 @default.
- W2061644855 hasConcept C2777292126 @default.
- W2061644855 hasConcept C2779409272 @default.
- W2061644855 hasConcept C4692481 @default.
- W2061644855 hasConcept C48023723 @default.
- W2061644855 hasConcept C530470458 @default.
- W2061644855 hasConcept C54355233 @default.
- W2061644855 hasConcept C56366318 @default.
- W2061644855 hasConcept C59822182 @default.
- W2061644855 hasConcept C84606932 @default.
- W2061644855 hasConcept C86803240 @default.
- W2061644855 hasConcept C95444343 @default.
- W2061644855 hasConceptScore W2061644855C104317684 @default.
- W2061644855 hasConceptScore W2061644855C105580179 @default.
- W2061644855 hasConceptScore W2061644855C121608353 @default.
- W2061644855 hasConceptScore W2061644855C125705527 @default.
- W2061644855 hasConceptScore W2061644855C153911025 @default.
- W2061644855 hasConceptScore W2061644855C196843134 @default.
- W2061644855 hasConceptScore W2061644855C2776415932 @default.
- W2061644855 hasConceptScore W2061644855C2776690073 @default.
- W2061644855 hasConceptScore W2061644855C2777292126 @default.
- W2061644855 hasConceptScore W2061644855C2779409272 @default.
- W2061644855 hasConceptScore W2061644855C4692481 @default.
- W2061644855 hasConceptScore W2061644855C48023723 @default.
- W2061644855 hasConceptScore W2061644855C530470458 @default.
- W2061644855 hasConceptScore W2061644855C54355233 @default.
- W2061644855 hasConceptScore W2061644855C56366318 @default.
- W2061644855 hasConceptScore W2061644855C59822182 @default.
- W2061644855 hasConceptScore W2061644855C84606932 @default.
- W2061644855 hasConceptScore W2061644855C86803240 @default.