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- W2061648265 abstract "Abstract A new computer program has been developed for the treatment of data from steroid radioimmunoassays. It uses an iterative technique that solves some 600 quadratic equations to compute the binding parameters for two independent, saturable binding agents from the standard curve data. The accuracy of curve fitting was such that all points on the standard curve were within one standard deviation of perfect fit, and no systematic bias was introduced into the results. Because the binding parameters were independent of the labelled ligand concentration, the methodology could be simplified, partially automated, and made more accurate and precise. The method was rapid, and 112 male plasma samples and 14 quality controls could be analysed (duplicate extracts on 40 μl aliquots with duplicate assays on each extract) by one person in one and a half days. The coefficient of variation between duplicate extracts of a reference plasma sample containing about 1000 ng testosterone/l00ml was 3.1% and the variation between assays on this sample conducted over a 3-month period was 3.8%." @default.
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- W2061648265 date "1976-10-01" @default.
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- W2061648265 title "A computerized semi-automated radioimmunoassay for plasma testosterone" @default.
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- W2061648265 doi "https://doi.org/10.1016/0022-4731(76)90189-8" @default.
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