FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W20616650> ?p ?o ?g. }

• W20616650 endingPage "8" @default.
• W20616650 startingPage "770" @default.
• W20616650 abstract "This study is aimed to develop a two-tube melting curve-based multiplex real time PCR assay (MCMRT-PCR) for the simultaneous detection of six common foodborne pathogenic bacteria (diarrhoeagenic Escherichia coli, Salmonella, and Shigella in tube 1, Staphylococcus aureus, Vibrio parahaemolyticus, and Listeria monocytogenes in tube 2).A two-tube MCMRT-PCR assay was performed on 7900HT Fast Real-Time PCR System (Applied Biosystems, USA). Amplification by PCR was optimized to obtain high efficiency. The sensitivity and specificity of assays were investigated.The detection limit of optimized MCMRT-PCR assay was 3.9×102 CFU/mL for S. aureus, 4.4×102 CFU/mL for L. monocytogenes, 3.0×102 CFU/mL for Salmonella, 2.5×102 CFU/mL for Shigella, 2.1×102 CFU/mL for V. parahaemolyticus, and 1.2×102 CFU/mL for E. coli. The feasibility of MCMRT-PCR was further evaluated using artificially contaminated milk, the sensitivity was at the level of 105 CFU/mL.A two-tube MCMRT-PCR assay using six primer sets was developed for detection of multiple pathogens. Our findings demonstrates that the proposed two-tube assay is reliable, useful and rapid for simultaneous detection of six foodborne pathogenic bacteria with an intended application in provincial Centers for Diseases Control and Prevention (CDC)." @default.
• W20616650 created "2016-06-24" @default.
• W20616650 creator A5003435653 @default.
• W20616650 creator A5018598237 @default.
• W20616650 creator A5034855502 @default.
• W20616650 creator A5043091074 @default.
• W20616650 creator A5044756931 @default.
• W20616650 date "2014-10-01" @default.
• W20616650 modified "2023-10-16" @default.
• W20616650 title "Detection and Identification of Six Foodborne Bacteria by Two-tube Multiplex Real Time PCR and Melting Curve Analysis." @default.
• W20616650 cites W117682463 @default.
• W20616650 cites W144754470 @default.
• W20616650 cites W1525752846 @default.
• W20616650 cites W1542632982 @default.
• W20616650 cites W1559932230 @default.
• W20616650 cites W1583376544 @default.
• W20616650 cites W1588194579 @default.
• W20616650 cites W1919891939 @default.
• W20616650 cites W1927918170 @default.
• W20616650 cites W1962223937 @default.
• W20616650 cites W1966615916 @default.
• W20616650 cites W1966850755 @default.
• W20616650 cites W1970019291 @default.
• W20616650 cites W1973016513 @default.
• W20616650 cites W1973946015 @default.
• W20616650 cites W1975758162 @default.
• W20616650 cites W1978783452 @default.
• W20616650 cites W1989922018 @default.
• W20616650 cites W1991859163 @default.
• W20616650 cites W1995295345 @default.
• W20616650 cites W2002277707 @default.
• W20616650 cites W2007724846 @default.
• W20616650 cites W2012248833 @default.
• W20616650 cites W2019820018 @default.
• W20616650 cites W2020373068 @default.
• W20616650 cites W2033084940 @default.
• W20616650 cites W2037148198 @default.
• W20616650 cites W2066077786 @default.
• W20616650 cites W2069199269 @default.
• W20616650 cites W2070529738 @default.
• W20616650 cites W2072376600 @default.
• W20616650 cites W2077806252 @default.
• W20616650 cites W2095655760 @default.
• W20616650 cites W2096654831 @default.
• W20616650 cites W2098916578 @default.
• W20616650 cites W2102786705 @default.
• W20616650 cites W2103798600 @default.
• W20616650 cites W2106303448 @default.
• W20616650 cites W2108278673 @default.
• W20616650 cites W2109132122 @default.
• W20616650 cites W2114998761 @default.
• W20616650 cites W2125983470 @default.
• W20616650 cites W2138152877 @default.
• W20616650 cites W2145853173 @default.
• W20616650 cites W2150705960 @default.
• W20616650 cites W2163062162 @default.
• W20616650 cites W2276794371 @default.
• W20616650 cites W2409261679 @default.
• W20616650 cites W54684614 @default.
• W20616650 cites W92128844 @default.
• W20616650 doi "https://doi.org/10.3967/bes2014.112" @default.
• W20616650 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25341812" @default.
• W20616650 hasPublicationYear "2014" @default.
• W20616650 type Work @default.
• W20616650 sameAs 20616650 @default.
• W20616650 citedByCount "1" @default.
• W20616650 countsByYear W206166502016 @default.
• W20616650 crossrefType "journal-article" @default.
• W20616650 hasAuthorship W20616650A5003435653 @default.
• W20616650 hasAuthorship W20616650A5018598237 @default.
• W20616650 hasAuthorship W20616650A5034855502 @default.
• W20616650 hasAuthorship W20616650A5043091074 @default.
• W20616650 hasAuthorship W20616650A5044756931 @default.
• W20616650 hasConcept C104317684 @default.
• W20616650 hasConcept C119128265 @default.
• W20616650 hasConcept C185592680 @default.
• W20616650 hasConcept C2776575720 @default.
• W20616650 hasConcept C2776986154 @default.
• W20616650 hasConcept C2778682378 @default.
• W20616650 hasConcept C2778883795 @default.
• W20616650 hasConcept C2779489039 @default.
• W20616650 hasConcept C2781065037 @default.
• W20616650 hasConcept C2781188995 @default.
• W20616650 hasConcept C2781350384 @default.
• W20616650 hasConcept C43617362 @default.
• W20616650 hasConcept C48023723 @default.
• W20616650 hasConcept C523546767 @default.
• W20616650 hasConcept C54355233 @default.
• W20616650 hasConcept C55493867 @default.
• W20616650 hasConcept C60644358 @default.
• W20616650 hasConcept C84133368 @default.
• W20616650 hasConcept C86803240 @default.
• W20616650 hasConcept C89423630 @default.
• W20616650 hasConceptScore W20616650C104317684 @default.
• W20616650 hasConceptScore W20616650C119128265 @default.
• W20616650 hasConceptScore W20616650C185592680 @default.
• W20616650 hasConceptScore W20616650C2776575720 @default.
• W20616650 hasConceptScore W20616650C2776986154 @default.

• next