Matches in SemOpenAlex for { <https://semopenalex.org/work/W2061749803> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2061749803 endingPage "530" @default.
- W2061749803 startingPage "515" @default.
- W2061749803 abstract "We have purified unadhered human monocytes in sufficient quantities to prepare monocyte plasma membrane vesicles and study vesicular calcium transport. Monocytes were isolated from plateletpheresis residues by counterflow centrifugal elutriation. By combining this source and procedure, 7 x 10(8) monocytes of over 90% purity were obtained. The membranes, isolated on a sucrose step gradient, had an 18-fold enrichment in Na,K-ATPase, a 29-fold diminution of succinate dehydrogenase activity and were vesicular on transmission electron micrographs. The membrane vesicles loaded with oxalate accumulated calcium only in the presence of Mg and ATP. Calcium uptake did not occur if ATP was replaced by any of five nucleotide phosphates or if Mg was omitted. Calcium transport had a maximal velocity of 4 pmoles calcium/micrograms vesicle protein/min and a Km for calcium of 0.53 microM. The ionophore A23187 completely inhibited calcium accumulation while 5 mM sodium cyanide and 10 microM ouabain had no effect. A calcium-activated ATPase was present in the same plasma membrane vesicles. The calcium ATPase had a maximal velocity of 18.0 pmoles calcium/micrograms vesicle protein/min and a Km for calcium of 0.60 microM. Calcium-activated ATPase activity was absent if Mg was omitted or if (gamma - 32P) GTP replaced (gamma - 32P) ATP. Monocyte plasma membranes that were stripped of endogenous calmodulin by EGTA treatment showed a reduced level of calcium uptake and calcium ATPase activity. The addition of exogenous calmodulin restored the transport activity to that of unstripped monocyte plasma membranes. Thus, monocyte plasma membrane vesicles contain a highly specific, ATP-dependent calcium transport system and a calcium-ATPase with similar high calcium affinities." @default.
- W2061749803 created "2016-06-24" @default.
- W2061749803 creator A5022401670 @default.
- W2061749803 creator A5082653656 @default.
- W2061749803 creator A5085473963 @default.
- W2061749803 date "1982-12-01" @default.
- W2061749803 modified "2023-09-27" @default.
- W2061749803 title "Plasma membrane vesicles prepared from unadhered monocytes: Characterization of calcium transport and the calcium ATPase" @default.
- W2061749803 cites W1570437501 @default.
- W2061749803 cites W1599011232 @default.
- W2061749803 cites W177553400 @default.
- W2061749803 cites W1775749144 @default.
- W2061749803 cites W1921593426 @default.
- W2061749803 cites W1974313407 @default.
- W2061749803 cites W2007126173 @default.
- W2061749803 cites W2029221759 @default.
- W2061749803 cites W2042204165 @default.
- W2061749803 cites W2050077668 @default.
- W2061749803 cites W2082553691 @default.
- W2061749803 cites W2090135458 @default.
- W2061749803 cites W2120944108 @default.
- W2061749803 cites W2140093238 @default.
- W2061749803 cites W2153847357 @default.
- W2061749803 cites W2160206263 @default.
- W2061749803 cites W2468684013 @default.
- W2061749803 cites W30889147 @default.
- W2061749803 cites W882800570 @default.
- W2061749803 doi "https://doi.org/10.1016/0143-4160(82)90042-2" @default.
- W2061749803 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6219742" @default.
- W2061749803 hasPublicationYear "1982" @default.
- W2061749803 type Work @default.
- W2061749803 sameAs 2061749803 @default.
- W2061749803 citedByCount "15" @default.
- W2061749803 countsByYear W20617498032017 @default.
- W2061749803 crossrefType "journal-article" @default.
- W2061749803 hasAuthorship W2061749803A5022401670 @default.
- W2061749803 hasAuthorship W2061749803A5082653656 @default.
- W2061749803 hasAuthorship W2061749803A5085473963 @default.
- W2061749803 hasConcept C101571972 @default.
- W2061749803 hasConcept C12554922 @default.
- W2061749803 hasConcept C130316041 @default.
- W2061749803 hasConcept C178790620 @default.
- W2061749803 hasConcept C181199279 @default.
- W2061749803 hasConcept C185592680 @default.
- W2061749803 hasConcept C23265538 @default.
- W2061749803 hasConcept C2777658833 @default.
- W2061749803 hasConcept C2779014961 @default.
- W2061749803 hasConcept C2779133857 @default.
- W2061749803 hasConcept C29688787 @default.
- W2061749803 hasConcept C41625074 @default.
- W2061749803 hasConcept C51705589 @default.
- W2061749803 hasConcept C519063684 @default.
- W2061749803 hasConcept C537181965 @default.
- W2061749803 hasConcept C55493867 @default.
- W2061749803 hasConcept C86803240 @default.
- W2061749803 hasConcept C935647 @default.
- W2061749803 hasConceptScore W2061749803C101571972 @default.
- W2061749803 hasConceptScore W2061749803C12554922 @default.
- W2061749803 hasConceptScore W2061749803C130316041 @default.
- W2061749803 hasConceptScore W2061749803C178790620 @default.
- W2061749803 hasConceptScore W2061749803C181199279 @default.
- W2061749803 hasConceptScore W2061749803C185592680 @default.
- W2061749803 hasConceptScore W2061749803C23265538 @default.
- W2061749803 hasConceptScore W2061749803C2777658833 @default.
- W2061749803 hasConceptScore W2061749803C2779014961 @default.
- W2061749803 hasConceptScore W2061749803C2779133857 @default.
- W2061749803 hasConceptScore W2061749803C29688787 @default.
- W2061749803 hasConceptScore W2061749803C41625074 @default.
- W2061749803 hasConceptScore W2061749803C51705589 @default.
- W2061749803 hasConceptScore W2061749803C519063684 @default.
- W2061749803 hasConceptScore W2061749803C537181965 @default.
- W2061749803 hasConceptScore W2061749803C55493867 @default.
- W2061749803 hasConceptScore W2061749803C86803240 @default.
- W2061749803 hasConceptScore W2061749803C935647 @default.
- W2061749803 hasIssue "6" @default.
- W2061749803 hasLocation W20617498031 @default.
- W2061749803 hasLocation W20617498032 @default.
- W2061749803 hasOpenAccess W2061749803 @default.
- W2061749803 hasPrimaryLocation W20617498031 @default.
- W2061749803 hasRelatedWork W1997130768 @default.
- W2061749803 hasRelatedWork W2016013368 @default.
- W2061749803 hasRelatedWork W2024094716 @default.
- W2061749803 hasRelatedWork W2025195721 @default.
- W2061749803 hasRelatedWork W2054125210 @default.
- W2061749803 hasRelatedWork W2058811387 @default.
- W2061749803 hasRelatedWork W2061749803 @default.
- W2061749803 hasRelatedWork W2083944173 @default.
- W2061749803 hasRelatedWork W298640429 @default.
- W2061749803 hasRelatedWork W87160560 @default.
- W2061749803 hasVolume "3" @default.
- W2061749803 isParatext "false" @default.
- W2061749803 isRetracted "false" @default.
- W2061749803 magId "2061749803" @default.
- W2061749803 workType "article" @default.