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- W2061796995 abstract "The EDTA titration of calcium using the change in fluorescence of Calcein indicator under ultraviolet illumination at the end-point was very satisfactory for essentially pure calcium chloride solutions. In order to use this method for biological samples, it was found best to separate the calcium by an oxalate precipitation at a pH of 4.7, convert the calcium oxalate to calcium carbonate, and dissolve the calcium carbonate in hydrochloric acid. Die EDTA-Titration von Calcium mittels fluorimetrischen Endpunkte unter Verwendung von UV-Licht und Calcein-indicator, verläuft in reinen Calciumlösungen sehr zufriedenstellend. Um diese Methode für biologische Proben heranzuziehen, wird Calcium erst als Oxalat gefällt, das Oxalat ins Karbonat übergeführt und letzteres in Salzsäure gelöst. Le titrage du calcium par L'EDTA utilisant la variation de fluorescence de l'indicateur, la calcéine, sous exposition ultra-violette au point équivalent, est très satisfaisant pour des solutions de chlorure de calcium pures. Afin d'utiliser cette méthode pour des échantillons biologiques, les auteurs ont trouvé qu'il était préférable de séparer le calcium par précipitation de Foxalate à pH 4,7, de transformer l'oxalate de calcium en carbonate de calcium et de dissoudre le carbonate de calcium dans l'acide chlorhydrique." @default.
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- W2061796995 date "1961-02-01" @default.
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- W2061796995 title "Determination of calcium in biological materialThe use of calcein as an indicator in the edta titration" @default.
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- W2061796995 doi "https://doi.org/10.1016/0039-9140(61)80015-5" @default.
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