FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2061797266> ?p ?o ?g. }

• W2061797266 endingPage "773" @default.
• W2061797266 startingPage "763" @default.
• W2061797266 abstract "The existence of two separate genetic lineages of Escherichia coli O157:H7 has previously been reported, and research indicates that lineage I could be more pathogenic toward human hosts than lineage II. We have previously shown that lineage I as a group expresses higher levels of Shiga toxin 2 (Stx2) than lineage II. To help evaluate why lineage II strains do not express appreciable levels of this toxin, whole-genome microarrays were performed using Agilent custom microarrays. Gene expression of the two representative bovine lineage II strains (FRIK966 and FRIK2000) were compared with gene expression of E. coli O157:H7 EDL933 (lineage I clinical type strain). Missing or differentially expressed genes and pathways were identified. Quantitative reverse transcription-polymerase chain reaction was performed to validate the microarray data. Draft genomes of FRIK966 and FRIK2000 were sequenced using Roche Applied Science/454 GS-FLX technology shotgun and paired-end approaches followed by de novo assembly. These assemblies were compared with the lineage I genome sequences from E. coli O157:H7 EDL933. The bacteriophage 933W, which encodes the Stx2 genes, showed a notable repression in gene expression. Polymerase chain reaction primers, based upon EDL933 genomic information, were also designed against all of the potentially missing genes of this bacteriophage. Most of the structural genes associated with the bacteriophage were found to be absent from the genome of the two bovine strains. These analyses, combined with evaluation of the genomic information, suggest that transposon (IS629) rearrangements may be associated with disruption of the bacteriophage genome in the FRIK strains. The results support the hypothesis that lineage II strains may be less of a risk as human foodborne pathogens. The microarray and genome data have been made available to the scientific community to allow continuing analysis of these cattle-isolated lineage II genomes and their gene expression." @default.
• W2061797266 created "2016-06-24" @default.
• W2061797266 creator A5007188678 @default.
• W2061797266 creator A5022478430 @default.
• W2061797266 creator A5023009331 @default.
• W2061797266 creator A5035817570 @default.
• W2061797266 creator A5035859456 @default.
• W2061797266 creator A5038992907 @default.
• W2061797266 creator A5047174631 @default.
• W2061797266 creator A5069573419 @default.
• W2061797266 creator A5074848128 @default.
• W2061797266 date "2010-07-01" @default.
• W2061797266 modified "2023-10-18" @default.
• W2061797266 title "Microarray Analysis and Draft Genomes of Two<i>Escherichia coli</i>O157:H7 Lineage II Cattle Isolates FRIK966 and FRIK2000 Investigating Lack of Shiga Toxin Expression" @default.
• W2061797266 cites W1560145325 @default.
• W2061797266 cites W1833131008 @default.
• W2061797266 cites W1885356084 @default.
• W2061797266 cites W1905377073 @default.
• W2061797266 cites W1920924307 @default.
• W2061797266 cites W1966533432 @default.
• W2061797266 cites W1967582134 @default.
• W2061797266 cites W1975057095 @default.
• W2061797266 cites W1980770184 @default.
• W2061797266 cites W1981521699 @default.
• W2061797266 cites W2010045259 @default.
• W2061797266 cites W2027078183 @default.
• W2061797266 cites W2032306900 @default.
• W2061797266 cites W2032402861 @default.
• W2061797266 cites W2039987645 @default.
• W2061797266 cites W2041107203 @default.
• W2061797266 cites W2045403483 @default.
• W2061797266 cites W2045765967 @default.
• W2061797266 cites W2066018712 @default.
• W2061797266 cites W2074484003 @default.
• W2061797266 cites W2077672202 @default.
• W2061797266 cites W2078689375 @default.
• W2061797266 cites W2083749072 @default.
• W2061797266 cites W2084326786 @default.
• W2061797266 cites W2096018607 @default.
• W2061797266 cites W2099836442 @default.
• W2061797266 cites W2103017472 @default.
• W2061797266 cites W2103798600 @default.
• W2061797266 cites W2108937299 @default.
• W2061797266 cites W2110739830 @default.
• W2061797266 cites W2115601595 @default.
• W2061797266 cites W2115698620 @default.
• W2061797266 cites W2122683221 @default.
• W2061797266 cites W2123629406 @default.
• W2061797266 cites W2127854361 @default.
• W2061797266 cites W2129323495 @default.
• W2061797266 cites W2134153749 @default.
• W2061797266 cites W2138959002 @default.
• W2061797266 cites W2150625556 @default.
• W2061797266 cites W2152244146 @default.
• W2061797266 cites W2159482845 @default.
• W2061797266 cites W2164505360 @default.
• W2061797266 cites W2164835198 @default.
• W2061797266 cites W2165233501 @default.
• W2061797266 cites W2167363775 @default.
• W2061797266 cites W2169267759 @default.
• W2061797266 cites W2170846767 @default.
• W2061797266 cites W2172709046 @default.
• W2061797266 cites W2218379543 @default.
• W2061797266 cites W2317729965 @default.
• W2061797266 cites W2410408684 @default.
• W2061797266 cites W4256409604 @default.
• W2061797266 doi "https://doi.org/10.1089/fpd.2009.0482" @default.
• W2061797266 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20156085" @default.
• W2061797266 hasPublicationYear "2010" @default.
• W2061797266 type Work @default.
• W2061797266 sameAs 2061797266 @default.
• W2061797266 citedByCount "14" @default.
• W2061797266 countsByYear W20617972662012 @default.
• W2061797266 countsByYear W20617972662013 @default.
• W2061797266 countsByYear W20617972662014 @default.
• W2061797266 countsByYear W20617972662015 @default.
• W2061797266 countsByYear W20617972662018 @default.
• W2061797266 countsByYear W20617972662019 @default.
• W2061797266 countsByYear W20617972662021 @default.
• W2061797266 crossrefType "journal-article" @default.
• W2061797266 hasAuthorship W2061797266A5007188678 @default.
• W2061797266 hasAuthorship W2061797266A5022478430 @default.
• W2061797266 hasAuthorship W2061797266A5023009331 @default.
• W2061797266 hasAuthorship W2061797266A5035817570 @default.
• W2061797266 hasAuthorship W2061797266A5035859456 @default.
• W2061797266 hasAuthorship W2061797266A5038992907 @default.
• W2061797266 hasAuthorship W2061797266A5047174631 @default.
• W2061797266 hasAuthorship W2061797266A5069573419 @default.
• W2061797266 hasAuthorship W2061797266A5074848128 @default.
• W2061797266 hasConcept C104317684 @default.
• W2061797266 hasConcept C141231307 @default.
• W2061797266 hasConcept C150194340 @default.
• W2061797266 hasConcept C2776441376 @default.
• W2061797266 hasConcept C2776817793 @default.
• W2061797266 hasConcept C2778411228 @default.
• W2061797266 hasConcept C2781001907 @default.
• W2061797266 hasConcept C54355233 @default.
• W2061797266 hasConcept C547475151 @default.

• next