Matches in SemOpenAlex for { <https://semopenalex.org/work/W2061804301> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2061804301 endingPage "260" @default.
- W2061804301 startingPage "249" @default.
- W2061804301 abstract "We have detected novel phosphotyrosine epitopes at the kinetochores of mitotic chromosomes in rat kangaroo PtK1 and mouse P388D1 tissue culture cells. Immunofluorescence labeling of detergent-resistant cytoskeletons reveals that these phosphotyrosine epitopes are tightly bound at the centrosomes and kinetochores of mitotic cells. These phosphoepitopes are found at the kinetochores during only prophase and prometaphase. Inclusion of a mixture of phosphatase inhibitors in the cell extraction procedure was necessary to preserve these previously undetected phosphotyrosine epitopes. The use of the phosphatase inhibitor mixture also improved the detection of the centrosome and kinetochore antigens recognized by the monoclonal antibody MPM-2. The MPM-2 antibody labels a subset of phosphothreonine-containing antigens found primarily during M phase. Ultrastructural immunolabeling studies indicated that both the phosphotyrosine and the MPM-2 phosphoepitopes were contained in both the outer and the inner dense plaques of the kinetochore. We developed large-scale chromosome isolation procedures designed to maintain chromosome protein phosphorylation. Immunoblot analysis revealed that the phosphotyrosine and MPM-2 antibodies recognized a number of chromosomal proteins, some of which were concentrated in the chromosome scaffold fraction prepared by nuclease digestion and salt extraction of whole chromosomes. The strictly regulated appearance of the phosphotyrosine and MPM-2 epitopes at the kinetochores of chromosomes during various stages of mitosis suggests that these phosphoepitopes may be involved in signal transduction pathways controlling kinetochore assembly and function during mitosis." @default.
- W2061804301 created "2016-06-24" @default.
- W2061804301 creator A5029799513 @default.
- W2061804301 creator A5054690860 @default.
- W2061804301 creator A5054956917 @default.
- W2061804301 date "1995-11-01" @default.
- W2061804301 modified "2023-09-26" @default.
- W2061804301 title "Cell-Cycle-Regulated Localization of Tyrosine and Threonine Phosphoepitopes at the Kinetochores of Mitotic Chromosomes" @default.
- W2061804301 doi "https://doi.org/10.1006/excr.1995.1373" @default.
- W2061804301 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7589252" @default.
- W2061804301 hasPublicationYear "1995" @default.
- W2061804301 type Work @default.
- W2061804301 sameAs 2061804301 @default.
- W2061804301 citedByCount "23" @default.
- W2061804301 countsByYear W20618043012015 @default.
- W2061804301 countsByYear W20618043012016 @default.
- W2061804301 countsByYear W20618043012017 @default.
- W2061804301 crossrefType "journal-article" @default.
- W2061804301 hasAuthorship W2061804301A5029799513 @default.
- W2061804301 hasAuthorship W2061804301A5054690860 @default.
- W2061804301 hasAuthorship W2061804301A5054956917 @default.
- W2061804301 hasConcept C104317684 @default.
- W2061804301 hasConcept C147483822 @default.
- W2061804301 hasConcept C1491633281 @default.
- W2061804301 hasConcept C153911025 @default.
- W2061804301 hasConcept C154202817 @default.
- W2061804301 hasConcept C178108356 @default.
- W2061804301 hasConcept C192838845 @default.
- W2061804301 hasConcept C195616568 @default.
- W2061804301 hasConcept C29537977 @default.
- W2061804301 hasConcept C30481170 @default.
- W2061804301 hasConcept C47432576 @default.
- W2061804301 hasConcept C54355233 @default.
- W2061804301 hasConcept C55493867 @default.
- W2061804301 hasConcept C63491729 @default.
- W2061804301 hasConcept C70278784 @default.
- W2061804301 hasConcept C85813293 @default.
- W2061804301 hasConcept C86803240 @default.
- W2061804301 hasConcept C93304396 @default.
- W2061804301 hasConcept C95444343 @default.
- W2061804301 hasConceptScore W2061804301C104317684 @default.
- W2061804301 hasConceptScore W2061804301C147483822 @default.
- W2061804301 hasConceptScore W2061804301C1491633281 @default.
- W2061804301 hasConceptScore W2061804301C153911025 @default.
- W2061804301 hasConceptScore W2061804301C154202817 @default.
- W2061804301 hasConceptScore W2061804301C178108356 @default.
- W2061804301 hasConceptScore W2061804301C192838845 @default.
- W2061804301 hasConceptScore W2061804301C195616568 @default.
- W2061804301 hasConceptScore W2061804301C29537977 @default.
- W2061804301 hasConceptScore W2061804301C30481170 @default.
- W2061804301 hasConceptScore W2061804301C47432576 @default.
- W2061804301 hasConceptScore W2061804301C54355233 @default.
- W2061804301 hasConceptScore W2061804301C55493867 @default.
- W2061804301 hasConceptScore W2061804301C63491729 @default.
- W2061804301 hasConceptScore W2061804301C70278784 @default.
- W2061804301 hasConceptScore W2061804301C85813293 @default.
- W2061804301 hasConceptScore W2061804301C86803240 @default.
- W2061804301 hasConceptScore W2061804301C93304396 @default.
- W2061804301 hasConceptScore W2061804301C95444343 @default.
- W2061804301 hasIssue "1" @default.
- W2061804301 hasLocation W20618043011 @default.
- W2061804301 hasLocation W20618043012 @default.
- W2061804301 hasOpenAccess W2061804301 @default.
- W2061804301 hasPrimaryLocation W20618043011 @default.
- W2061804301 hasRelatedWork W1956419734 @default.
- W2061804301 hasRelatedWork W1965047055 @default.
- W2061804301 hasRelatedWork W2011054691 @default.
- W2061804301 hasRelatedWork W2092737854 @default.
- W2061804301 hasRelatedWork W2125430425 @default.
- W2061804301 hasRelatedWork W2143773230 @default.
- W2061804301 hasRelatedWork W2159636699 @default.
- W2061804301 hasRelatedWork W2409106442 @default.
- W2061804301 hasRelatedWork W2899676732 @default.
- W2061804301 hasRelatedWork W4247847006 @default.
- W2061804301 hasVolume "221" @default.
- W2061804301 isParatext "false" @default.
- W2061804301 isRetracted "false" @default.
- W2061804301 magId "2061804301" @default.
- W2061804301 workType "article" @default.