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• W2061822834 abstract "Fibroblasts cultured from normal human forearm or genital skin were exposed to dexamethasone (DM) 10(-9)-10(-7) M, deoxycorticosterone (DOC) 10(-6)M, or vehicle for 4-16 h and [35S]methionine-labelled proteins compared by two-dimensional gel electrophoresis. No effects on cell number or [35S]methionine incorporation were seen with either steroid, nor any effects of DOC on patterns of protein synthesis. In contrast, after 4 h incubation DM consistently (24/24 gels) caused increased abundance of a protein spot designated k (kappa; Mr approximately 41 000, pKi approximately 6.5). After 16 h steroid exposure, levels of protein kappa remained elevated; abundance of a second protein spot omega (omega; Mr approximately 42 000, pKi approximately 5.4) was consistently (8/8 gels) lower than in control fibroblasts. We interpret these data as evidence for glucocorticoid-specific effects on human skin fibroblasts, and that (within the pKi and molecular weight range studied) the observed changes in abundance of proteins kappa and omega constitute the human fibroblast glucocorticoid domain." @default.
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• W2061822834 date "1983-06-01" @default.
• W2061822834 modified "2023-09-29" @default.
• W2061822834 title "Effects of steroids on fibroblasts: Identification of glucocorticoid-regulated proteins by two-dimensional gel electrophoresis" @default.
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• W2061822834 doi "https://doi.org/10.1016/0303-7207(83)90066-7" @default.
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