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- W2061837197 abstract "Lens epithelial cells from neonatal rats cultured with neural retinas or neural retina-conditioned medium (RCM), undergo fibre differentiation. This is characterized by cell elongation, increased α-crystallin synthesis and the initiation of β and γ-crystallin synthesis. To determine if this tissue interaction continues in later life we developed an ELISA method to analyse patterns of α and β-crystallin accumulation in epithelia from 3-day-, 10-day- and 21-day-old rats. Culture of lens epithelia with RCM resulted in the formation of multilayers of elongated fibres and the accumulation of α and β-crystallins. The patterns of crystallin accumulation were essentially similar whether expressed as μg crystallin per explant, or crystallin per DNA (ng per ng). α- and β-Crystallins accumulated rapidly in explants after 2 days of culture in RCM, whereas explants grown in control medium showed no change in the crystallin levels from day 0 to day 10. Patterns of α- and β-crystallin accumulation showed that there were no significant differences between the ability of lens epithelia from 3-day-, 10-day or 21-day-old rats to undergo fibre differentiation in response to RCM. Therefore we conclude that the inductive interaction between lens and neural retina is not restricted to embryonic or neonatal stages, but continues on throughout life maintaining normal patterns of fibre differentiation in the lens." @default.
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- W2061837197 date "1986-12-01" @default.
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- W2061837197 title "Analysis of an inductive interaction between lens and neural retina in rats of different ages" @default.
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- W2061837197 doi "https://doi.org/10.1016/0014-4835(86)90080-1" @default.
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