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- W2061856241 abstract "Abstract In this novel enzyme-tagged immunoelectrochemical assay, disposable carbon felt discs serve both as electrodes and as the heterogeneous solid phase. Antibodies are immobilized on the carbon felt via a diaminoalkane-biotin-avidin-biotin bridge. Alkaline phosphatase is used as a label. Bound antibodies are monitored by following the electro-oxidation of aminophenol, produced enzymatically from p-amino-phenyl phosphate by the immobilized alkaline phosphatase at the electrode surface. A model system designed for determination of mouse IgG concentration yielded a calibration curve ranging from 10pg/ml to 100ug/ml. This assay can be performed rapidly and a single determination completed within 20 minutes. The system is useful also for rapid quantitation of a small number (∼80 organisms per ml) of bacteria." @default.
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- W2061856241 date "1992-06-01" @default.
- W2061856241 modified "2023-09-27" @default.
- W2061856241 title "A Rapid and Sensitive Heterogeneous Immunoelectrochemical Assay Using Disposable Electrodes" @default.
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- W2061856241 doi "https://doi.org/10.1080/15321819208021229" @default.
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