FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2061874162> ?p ?o ?g. }

• W2061874162 endingPage "1377" @default.
• W2061874162 startingPage "1366" @default.
• W2061874162 abstract "Quantitative proteomics is challenging and various stable isotope based approaches have been developed to meet the challenge. Hereby we describe a simple, efficient, reliable, and inexpensive method named reductive alkylation by acetone (RABA) to introduce stable isotopes to peptides for quantitative analysis. The RABA method leads to alkylation of N-terminal and lysine amino groups with isopropyl moiety. Using unlabeled (d0) and deuterium labeled (d6) acetone, a 6 Da mass split is introduced to each isopropyl modification between the light and heavy isotope labeled peptides, which is ideally suited for quantitative analysis. The reaction specificity, stoichiometry, labeling efficiency, and linear range of the RABA method have been thoroughly evaluated in this study using standard peptides, tryptic digest of proteins, as well as human cell lysate. Reliable quantitative results have been consistently obtained in all experiments. We also applied the RABA method to quantitative analysis of proteins in spinal cords of transgenic mouse models of amyotrophic lateral sclerosis. Highly homologous proteins (transgenic human SOD1 and endogenous mouse SOD1) were distinguished and quantified using the method developed in this study. In addition, the quantitative results using the RABA approach were independently validated by Western blot." @default.
• W2061874162 created "2016-06-24" @default.
• W2061874162 creator A5004349988 @default.
• W2061874162 creator A5031518529 @default.
• W2061874162 creator A5037143610 @default.
• W2061874162 creator A5083251487 @default.
• W2061874162 date "2009-07-01" @default.
• W2061874162 modified "2023-10-12" @default.
• W2061874162 title "RABA (Reductive Alkylation By Acetone): A novel stable isotope labeling approach for quantitative proteomics" @default.
• W2061874162 cites W1597883912 @default.
• W2061874162 cites W1797925867 @default.
• W2061874162 cites W1965155369 @default.
• W2061874162 cites W1972055109 @default.
• W2061874162 cites W1974849210 @default.
• W2061874162 cites W1975014695 @default.
• W2061874162 cites W1975938671 @default.
• W2061874162 cites W1986810516 @default.
• W2061874162 cites W1992843897 @default.
• W2061874162 cites W1996063525 @default.
• W2061874162 cites W1997898173 @default.
• W2061874162 cites W2006419756 @default.
• W2061874162 cites W2014725206 @default.
• W2061874162 cites W2017678299 @default.
• W2061874162 cites W2023096047 @default.
• W2061874162 cites W2025422229 @default.
• W2061874162 cites W2028445484 @default.
• W2061874162 cites W2029220511 @default.
• W2061874162 cites W2031226474 @default.
• W2061874162 cites W2036741271 @default.
• W2061874162 cites W2045303955 @default.
• W2061874162 cites W2048333869 @default.
• W2061874162 cites W2057139034 @default.
• W2061874162 cites W2059179399 @default.
• W2061874162 cites W2062017840 @default.
• W2061874162 cites W2069862631 @default.
• W2061874162 cites W2076267319 @default.
• W2061874162 cites W2078662681 @default.
• W2061874162 cites W2086216615 @default.
• W2061874162 cites W2088392539 @default.
• W2061874162 cites W2091619455 @default.
• W2061874162 cites W2097785509 @default.
• W2061874162 cites W2106187536 @default.
• W2061874162 cites W2111208057 @default.
• W2061874162 cites W2112496677 @default.
• W2061874162 cites W2115756856 @default.
• W2061874162 cites W2120016245 @default.
• W2061874162 cites W2124711900 @default.
• W2061874162 cites W2127526794 @default.
• W2061874162 cites W2140946052 @default.
• W2061874162 cites W2144718087 @default.
• W2061874162 cites W2163021396 @default.
• W2061874162 doi "https://doi.org/10.1016/j.jasms.2009.03.027" @default.
• W2061874162 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2731973" @default.
• W2061874162 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19419886" @default.
• W2061874162 hasPublicationYear "2009" @default.
• W2061874162 type Work @default.
• W2061874162 sameAs 2061874162 @default.
• W2061874162 citedByCount "16" @default.
• W2061874162 countsByYear W20618741622012 @default.
• W2061874162 countsByYear W20618741622013 @default.
• W2061874162 countsByYear W20618741622016 @default.
• W2061874162 countsByYear W20618741622020 @default.
• W2061874162 countsByYear W20618741622021 @default.
• W2061874162 countsByYear W20618741622022 @default.
• W2061874162 crossrefType "journal-article" @default.
• W2061874162 hasAuthorship W2061874162A5004349988 @default.
• W2061874162 hasAuthorship W2061874162A5031518529 @default.
• W2061874162 hasAuthorship W2061874162A5037143610 @default.
• W2061874162 hasAuthorship W2061874162A5083251487 @default.
• W2061874162 hasBestOaLocation W20618741621 @default.
• W2061874162 hasConcept C104317684 @default.
• W2061874162 hasConcept C162356407 @default.
• W2061874162 hasConcept C185592680 @default.
• W2061874162 hasConcept C2776415932 @default.
• W2061874162 hasConcept C43617362 @default.
• W2061874162 hasConcept C46111723 @default.
• W2061874162 hasConcept C55493867 @default.
• W2061874162 hasConcept C80311884 @default.
• W2061874162 hasConcept C95986675 @default.
• W2061874162 hasConceptScore W2061874162C104317684 @default.
• W2061874162 hasConceptScore W2061874162C162356407 @default.
• W2061874162 hasConceptScore W2061874162C185592680 @default.
• W2061874162 hasConceptScore W2061874162C2776415932 @default.
• W2061874162 hasConceptScore W2061874162C43617362 @default.
• W2061874162 hasConceptScore W2061874162C46111723 @default.
• W2061874162 hasConceptScore W2061874162C55493867 @default.
• W2061874162 hasConceptScore W2061874162C80311884 @default.
• W2061874162 hasConceptScore W2061874162C95986675 @default.
• W2061874162 hasIssue "7" @default.
• W2061874162 hasLocation W20618741621 @default.
• W2061874162 hasLocation W20618741622 @default.
• W2061874162 hasLocation W20618741623 @default.
• W2061874162 hasLocation W20618741624 @default.
• W2061874162 hasOpenAccess W2061874162 @default.
• W2061874162 hasPrimaryLocation W20618741621 @default.
• W2061874162 hasRelatedWork W111275185 @default.
• W2061874162 hasRelatedWork W1975323042 @default.
• W2061874162 hasRelatedWork W1975558168 @default.

• next