FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2061876382> ?p ?o ?g. }

• W2061876382 abstract "Cholesterol content of cells must be maintained within the very tight limits, too much or too little cholesterol in a cell results in disruption of cellular membranes, apoptosis and necrosis. Cells can source cholesterol from intracellular synthesis and from plasma lipoproteins, both sources are sufficient to fully satisfy cells' requirements for cholesterol. The processes of cholesterol synthesis and uptake are tightly regulated and deficiencies of cholesterol are rare. Excessive cholesterol is more common problem. With the exception of hepatocytes and to some degree adrenocortical cells, cells are unable to degrade cholesterol. Cells have two options to reduce their cholesterol content: to convert cholesterol into cholesteryl esters, an option with limited capacity as overloading cells with cholesteryl esters is also toxic, and cholesterol efflux, an option with potentially unlimited capacity. Cholesterol efflux is a specific process that is regulated by a number of intracellular transporters, such as ATP binding cassette transporter proteins A1 (ABCA1) and G1 (ABCG1) and scavenger receptor type B1. The natural acceptor of cholesterol in plasma is high density lipoprotein (HDL) and apolipoprotein A-I. The cholesterol efflux assay is designed to quantitate the rate of cholesterol efflux from cultured cells. It measures the capacity of cells to maintain cholesterol efflux and/or the capacity of plasma acceptors to accept cholesterol released from cells. The assay consists of the following steps. Step 1: labelling cellular cholesterol by adding labelled cholesterol to serum-containing medium and incubating with cells for 24-48 h. This step may be combined with loading of cells with cholesterol. Step 2: incubation of cells in serum-free medium to equilibrate labelled cholesterol among all intracellular cholesterol pools. This stage may be combined with activation of cellular cholesterol transporters. Step 3: incubation of cells with extracellular acceptor and quantitation of movement of labelled cholesterol from cells to the acceptor. If cholesterol precursors were used to label newly synthesized cholesterol, a fourth step, purification of cholesterol, may be required. The assay delivers the following information: (i) how a particular treatment (a mutation, a knock-down, an overexpression or a treatment) affects the capacity of cell to efflux cholesterol and (ii) how the capacity of plasma acceptors to accept cholesterol is affected by a disease or a treatment. This method is often used in context of cardiovascular research, metabolic and neurodegenerative disorders, infectious and reproductive diseases." @default.
• W2061876382 created "2016-06-24" @default.
• W2061876382 creator A5049870943 @default.
• W2061876382 creator A5069825390 @default.
• W2061876382 creator A5087570445 @default.
• W2061876382 date "2012-03-06" @default.
• W2061876382 modified "2023-10-12" @default.
• W2061876382 title "Cholesterol Efflux Assay" @default.
• W2061876382 cites W1513634422 @default.
• W2061876382 cites W1977459863 @default.
• W2061876382 cites W1992565781 @default.
• W2061876382 cites W1998486318 @default.
• W2061876382 cites W2020988004 @default.
• W2061876382 cites W2022806912 @default.
• W2061876382 cites W2046415613 @default.
• W2061876382 cites W2062160450 @default.
• W2061876382 cites W2073185340 @default.
• W2061876382 cites W2107088295 @default.
• W2061876382 cites W2110960044 @default.
• W2061876382 cites W2112262627 @default.
• W2061876382 cites W2114991100 @default.
• W2061876382 cites W2148789868 @default.
• W2061876382 cites W2150680144 @default.
• W2061876382 cites W2169501009 @default.
• W2061876382 doi "https://doi.org/10.3791/3810" @default.
• W2061876382 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3460567" @default.
• W2061876382 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22414908" @default.
• W2061876382 hasPublicationYear "2012" @default.
• W2061876382 type Work @default.
• W2061876382 sameAs 2061876382 @default.
• W2061876382 citedByCount "44" @default.
• W2061876382 countsByYear W20618763822013 @default.
• W2061876382 countsByYear W20618763822014 @default.
• W2061876382 countsByYear W20618763822015 @default.
• W2061876382 countsByYear W20618763822016 @default.
• W2061876382 countsByYear W20618763822017 @default.
• W2061876382 countsByYear W20618763822018 @default.
• W2061876382 countsByYear W20618763822019 @default.
• W2061876382 countsByYear W20618763822020 @default.
• W2061876382 countsByYear W20618763822021 @default.
• W2061876382 countsByYear W20618763822022 @default.
• W2061876382 countsByYear W20618763822023 @default.
• W2061876382 crossrefType "journal-article" @default.
• W2061876382 hasAuthorship W2061876382A5049870943 @default.
• W2061876382 hasAuthorship W2061876382A5069825390 @default.
• W2061876382 hasAuthorship W2061876382A5087570445 @default.
• W2061876382 hasBestOaLocation W20618763821 @default.
• W2061876382 hasConcept C104317684 @default.
• W2061876382 hasConcept C110459506 @default.
• W2061876382 hasConcept C149011108 @default.
• W2061876382 hasConcept C185592680 @default.
• W2061876382 hasConcept C200082930 @default.
• W2061876382 hasConcept C2777704780 @default.
• W2061876382 hasConcept C2778163477 @default.
• W2061876382 hasConcept C2780072125 @default.
• W2061876382 hasConcept C2910322942 @default.
• W2061876382 hasConcept C41443022 @default.
• W2061876382 hasConcept C55493867 @default.
• W2061876382 hasConcept C86803240 @default.
• W2061876382 hasConcept C95444343 @default.
• W2061876382 hasConceptScore W2061876382C104317684 @default.
• W2061876382 hasConceptScore W2061876382C110459506 @default.
• W2061876382 hasConceptScore W2061876382C149011108 @default.
• W2061876382 hasConceptScore W2061876382C185592680 @default.
• W2061876382 hasConceptScore W2061876382C200082930 @default.
• W2061876382 hasConceptScore W2061876382C2777704780 @default.
• W2061876382 hasConceptScore W2061876382C2778163477 @default.
• W2061876382 hasConceptScore W2061876382C2780072125 @default.
• W2061876382 hasConceptScore W2061876382C2910322942 @default.
• W2061876382 hasConceptScore W2061876382C41443022 @default.
• W2061876382 hasConceptScore W2061876382C55493867 @default.
• W2061876382 hasConceptScore W2061876382C86803240 @default.
• W2061876382 hasConceptScore W2061876382C95444343 @default.
• W2061876382 hasIssue "61" @default.
• W2061876382 hasLocation W20618763821 @default.
• W2061876382 hasLocation W20618763822 @default.
• W2061876382 hasLocation W20618763823 @default.
• W2061876382 hasLocation W20618763824 @default.
• W2061876382 hasOpenAccess W2061876382 @default.
• W2061876382 hasPrimaryLocation W20618763821 @default.
• W2061876382 hasRelatedWork W1780633743 @default.
• W2061876382 hasRelatedWork W2026956555 @default.
• W2061876382 hasRelatedWork W2030703179 @default.
• W2061876382 hasRelatedWork W2074851385 @default.
• W2061876382 hasRelatedWork W2108608979 @default.
• W2061876382 hasRelatedWork W2111482014 @default.
• W2061876382 hasRelatedWork W2123241855 @default.
• W2061876382 hasRelatedWork W2219715151 @default.
• W2061876382 hasRelatedWork W2396513904 @default.
• W2061876382 hasRelatedWork W2884851592 @default.
• W2061876382 isParatext "false" @default.
• W2061876382 isRetracted "false" @default.
• W2061876382 magId "2061876382" @default.
• W2061876382 workType "article" @default.