Matches in SemOpenAlex for { <https://semopenalex.org/work/W2061947192> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2061947192 endingPage "489" @default.
- W2061947192 startingPage "485" @default.
- W2061947192 abstract "We have cloned and sequenced cDNAs encoding the variable regions of the light (VL) and heavy (VH) chains of monoclonal antibody KC4G3. VL belongs to group II and resulted from a Vκ-Jκ2 recombination. VH belongs to group IIId and arose from a V-D9-JH3 recombination. The VL and VH frameworks are respectively 84% and 83% identical to the corresponding VL and VH human consensus frameworks. The deduced amino acid sequence of VL contains an asparagine-linked glycosylation site in framework 3 (N74 I75 S76). We have determined that a large fraction of the light chains are indeed glycolysated. We constructed an IgG1,κ human/mouse chimeric antibody (by inserting the murine KC4G3 Fv-encoding cDNAs into plasmids encoding a human IgG1,κ Fc domain) and expressed it in SP2/0-Ag14 mouse myeloma cells. This chimeric monoclonal antibody is designated ChiKC4. We have determined that the murine monoclonal antibody KC4G3 binds the human breast mucin with an affinity constant of 1.1×l09 M-1. ChiKC4 binds the same antigen with an affinity constant of 1.2×109 M-1. ChiKC4 stains breast carcinoma tissue sections by the ABC immunoperoxidase method in an identical manner as does KC4G3." @default.
- W2061947192 created "2016-06-24" @default.
- W2061947192 creator A5014151044 @default.
- W2061947192 creator A5020981900 @default.
- W2061947192 creator A5046199922 @default.
- W2061947192 creator A5090172616 @default.
- W2061947192 date "1993-08-01" @default.
- W2061947192 modified "2023-09-25" @default.
- W2061947192 title "Cloning of cDNAs Encoding the Variable Domains of Antibody KC4G3 and Construction of a Chimeric Antibody" @default.
- W2061947192 cites W13170960 @default.
- W2061947192 cites W134369158 @default.
- W2061947192 cites W1483404226 @default.
- W2061947192 cites W177536608 @default.
- W2061947192 cites W1939186328 @default.
- W2061947192 cites W1963635544 @default.
- W2061947192 cites W1974869964 @default.
- W2061947192 cites W1977461031 @default.
- W2061947192 cites W1980651107 @default.
- W2061947192 cites W1980826960 @default.
- W2061947192 cites W1981955875 @default.
- W2061947192 cites W1983253653 @default.
- W2061947192 cites W1983494459 @default.
- W2061947192 cites W1988512414 @default.
- W2061947192 cites W2009105413 @default.
- W2061947192 cites W2017381373 @default.
- W2061947192 cites W2026763202 @default.
- W2061947192 cites W2032144081 @default.
- W2061947192 cites W2040505385 @default.
- W2061947192 cites W2040650320 @default.
- W2061947192 cites W2051330838 @default.
- W2061947192 cites W2054845377 @default.
- W2061947192 cites W2079261085 @default.
- W2061947192 cites W2091850539 @default.
- W2061947192 cites W2097005775 @default.
- W2061947192 cites W2101545320 @default.
- W2061947192 cites W2172041865 @default.
- W2061947192 cites W2290754814 @default.
- W2061947192 cites W2483634850 @default.
- W2061947192 doi "https://doi.org/10.1089/hyb.1993.12.485" @default.
- W2061947192 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8454302" @default.
- W2061947192 hasPublicationYear "1993" @default.
- W2061947192 type Work @default.
- W2061947192 sameAs 2061947192 @default.
- W2061947192 citedByCount "7" @default.
- W2061947192 countsByYear W20619471922013 @default.
- W2061947192 countsByYear W20619471922017 @default.
- W2061947192 crossrefType "journal-article" @default.
- W2061947192 hasAuthorship W2061947192A5014151044 @default.
- W2061947192 hasAuthorship W2061947192A5020981900 @default.
- W2061947192 hasAuthorship W2061947192A5046199922 @default.
- W2061947192 hasAuthorship W2061947192A5090172616 @default.
- W2061947192 hasConcept C104317684 @default.
- W2061947192 hasConcept C123894998 @default.
- W2061947192 hasConcept C153911025 @default.
- W2061947192 hasConcept C159654299 @default.
- W2061947192 hasConcept C167625842 @default.
- W2061947192 hasConcept C187882448 @default.
- W2061947192 hasConcept C36394416 @default.
- W2061947192 hasConcept C40767141 @default.
- W2061947192 hasConcept C542903549 @default.
- W2061947192 hasConcept C54355233 @default.
- W2061947192 hasConcept C86803240 @default.
- W2061947192 hasConceptScore W2061947192C104317684 @default.
- W2061947192 hasConceptScore W2061947192C123894998 @default.
- W2061947192 hasConceptScore W2061947192C153911025 @default.
- W2061947192 hasConceptScore W2061947192C159654299 @default.
- W2061947192 hasConceptScore W2061947192C167625842 @default.
- W2061947192 hasConceptScore W2061947192C187882448 @default.
- W2061947192 hasConceptScore W2061947192C36394416 @default.
- W2061947192 hasConceptScore W2061947192C40767141 @default.
- W2061947192 hasConceptScore W2061947192C542903549 @default.
- W2061947192 hasConceptScore W2061947192C54355233 @default.
- W2061947192 hasConceptScore W2061947192C86803240 @default.
- W2061947192 hasIssue "4" @default.
- W2061947192 hasLocation W20619471921 @default.
- W2061947192 hasLocation W20619471922 @default.
- W2061947192 hasOpenAccess W2061947192 @default.
- W2061947192 hasPrimaryLocation W20619471921 @default.
- W2061947192 hasRelatedWork W2055737043 @default.
- W2061947192 hasRelatedWork W2083958575 @default.
- W2061947192 hasRelatedWork W2122212633 @default.
- W2061947192 hasRelatedWork W2355498255 @default.
- W2061947192 hasRelatedWork W2380405330 @default.
- W2061947192 hasRelatedWork W2380817849 @default.
- W2061947192 hasRelatedWork W2389269354 @default.
- W2061947192 hasRelatedWork W2393170758 @default.
- W2061947192 hasRelatedWork W2417074343 @default.
- W2061947192 hasRelatedWork W2429425663 @default.
- W2061947192 hasVolume "12" @default.
- W2061947192 isParatext "false" @default.
- W2061947192 isRetracted "false" @default.
- W2061947192 magId "2061947192" @default.
- W2061947192 workType "article" @default.