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• W2061965359 abstract "The bacterial quality and safety of raw milk sources in Taif region (Western Saudi Arabia) were analyzed for the natural contamination of fecal coliform and Escherichia coli by standard most probable number method. The E. coli isolates were identified to species identity using API 20E and screened for markers of STEC (Stx1, Stx2), ETEC (ST, LT), (EaeA) and ExPEC (CNF1, CNF2, SfaS, PapA, CdtB, IutA, FyuA, TraT) using PCR assays. Thirty-three E. coli strains were recovered from raw milk sample sources, which were contaminated by fecal coliform. None of the investigated isolates reacted positively with the PapA, SfaS and CdtB, ST and LT-specific primers. Markers of STEC and EPEC were each detected in three strains. NTEC was detected in four strains. The most frequent virulence markers were TraT (17 strains), IutA (11 strains) and FyuA (8 strains). Results suggested a possibility of potential public health threat of E. coli originating from raw milk sources." @default.
• W2061965359 created "2016-06-24" @default.
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• W2061965359 date "2009-10-01" @default.
• W2061965359 modified "2023-10-03" @default.
• W2061965359 title "Bacterial quality of raw milk investigated by Escherichia coli and isolates analysis for specific virulence-gene markers" @default.
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• W2061965359 doi "https://doi.org/10.1016/j.foodcont.2009.01.005" @default.
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