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- W2062002191 abstract "We studied the distribution of α-amylase mRNA in normal dog tissues by northern blotting (NB) and reverse transcription-polymerase chain reaction (RT PCR) with human pancreatic (AMV2) and salivary (AMY1) α-amylase cDNA-specific primers. Analysis of poly(A+) RNA from various normal tissues by NB indicated the presence of detectable levels of α-amylase mRNA transcripts only in pancreas. Dot-blot analysis of DNA amplified with primers common to both (human) isoamylase mRNAs showed presence of α-amylase gene transcripts not only In pancreas but also in liver, small Intestine, large Intestine and fallopian tube. Traces of amylase gene transcripts were also observed in ovary, uterus and lung. Interestingly, amylase transcripts were not detectable in the parotid gland by NB or RT-PCR. We have also localized α-amylase mRNA transcripts to dog pancreas by in situ transcription and in situ hybridization. Our results suggest that there Is high degree of homology between the a-amylase mRNA sequences in dog and human at least in the exon 3–4 regions of the human gene." @default.
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- W2062002191 date "1990-01-01" @default.
- W2062002191 modified "2023-10-14" @default.
- W2062002191 title "α-Amylase gene transcription in tissues of normal dog" @default.
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- W2062002191 doi "https://doi.org/10.1093/nar/18.4.1031" @default.
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