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• W2062013337 abstract "In this paper, we provide an update on O2-dependent membrane transport in red cells. O2-sensitive membrane transport was compared in nucleated (chicken) and enucleated (human) red cells, to investigate effects on organic (glucose transporter [GLUT]) and inorganic (K+–Cl− cotransporter [KCC]/Na+–K+–2Cl− cotransporter [NKCC]) transporters, to study the response of so-called “housekeeping” transporters (Na+/K+ pump and anion exchanger [AE]) and, finally, to compare O2 sensitivity in normal human red cells with those from sickle cell patients. The Na+/K+ pump showed no change in activity between oxygenated and deoxygenated cells in any of the samples. KCC in normal human red cells had the greatest O2 sensitivity, being stimulated some 20-fold on oxygenation. It was more modestly stimulated by O2 in chicken red cells and HbS cells. By contrast, NKCC was stimulated by deoxygenation in all cases. GLUT showed little response to O2 tension, other than a small stimulation in deoxygenated chicken red cells. Finally, AE1 was stimulated by oxygenation in HbA cells, but this stimulation by O2 was absent in HbS cells and pink ghosts prepared from HbA cells. The significance of these findings is discussed." @default.
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• W2062013337 date "2004-05-01" @default.
• W2062013337 modified "2023-10-11" @default.
• W2062013337 title "Oxygen sensitivity of red cell membrane transporters revisited" @default.
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• W2062013337 doi "https://doi.org/10.1016/j.bioelechem.2003.07.003" @default.
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