Matches in SemOpenAlex for { <https://semopenalex.org/work/W2062025503> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2062025503 endingPage "74" @default.
- W2062025503 startingPage "71" @default.
- W2062025503 abstract "The plasminogen activation (PA) system is involved in vascular remodelling. Modulating its activity in vascular cells might be a way to interfere in processes such as angiogenesis and restenosis. Adenoviral vectors have become a favourable tool for direct gene transfer into vascular cells. In the interest of using adenoviral vectors to modulate plasminogen activator activity and endothelial and smooth muscle cell migration, we studied the effects of endothelial and smooth muscle cell transduction in vitro and in the umbilical vein ex vivo with a replication-defective adenoviral vector containing the β-galactosidase gene (AdCMVLacZ). Segments of the umbilical vein were infected with AdCMVLacZ (109–1010 pfu/ml). After 48 h strong β-galactosidase expression could be observed in the vessel wall, which was restricted to the endothelial layer. Although some heterogeneity in the transduction throughout the vessel could be seen, β-galactosidase expression was detectable for 21 days in explant. Infection of human vascular endothelial cells (HUVEC) with recombinant adenoviral vectors is a dose and time dependent process. To achieve 100% infection of cultured HUVEC after a 30 min infection period, adenovirus concentrations ranging from 1010 to 5·1010 pfu/ml are required. To achieve a similar infection of HUVSMC a concentration of 2·109 to 1010 is sufficient. Expression of β-galactosidase can be detected for at least 14 days. Effects of transduction of HUVEC with AdCMVLacZ on proliferation, morphology and monolayer integrity were also studied. At high virus concentrations (> 1010 pfu/ml) an inhibitory effect on cell proliferation was detected and cell morphology displayed many giant cells, often polynuclear, in these cultures. Infection of confluent HUVEC monolayers had, apart from a rapid transient effect during the infection procedure, little effect on the permeability of these monolayers. Only when the monolayers were infected using concentrations of 1010 pfu/ml or more, an increase in permeability of these monolayers could be observed." @default.
- W2062025503 created "2016-06-24" @default.
- W2062025503 creator A5002064223 @default.
- W2062025503 creator A5005383720 @default.
- W2062025503 creator A5015793061 @default.
- W2062025503 creator A5042649357 @default.
- W2062025503 creator A5056821715 @default.
- W2062025503 creator A5068113965 @default.
- W2062025503 creator A5083354719 @default.
- W2062025503 date "1996-06-01" @default.
- W2062025503 modified "2023-10-11" @default.
- W2062025503 title "Effects of infection with recombinant adenovirus on human vascular endothelial and smooth muscle cells" @default.
- W2062025503 cites W1965295123 @default.
- W2062025503 cites W1992705822 @default.
- W2062025503 cites W2017614092 @default.
- W2062025503 cites W2021880042 @default.
- W2062025503 cites W2043558120 @default.
- W2062025503 cites W2047561204 @default.
- W2062025503 cites W2048774847 @default.
- W2062025503 cites W2052612816 @default.
- W2062025503 cites W2167514893 @default.
- W2062025503 cites W4300379097 @default.
- W2062025503 doi "https://doi.org/10.1016/s0268-9499(96)80054-3" @default.
- W2062025503 hasPublicationYear "1996" @default.
- W2062025503 type Work @default.
- W2062025503 sameAs 2062025503 @default.
- W2062025503 citedByCount "1" @default.
- W2062025503 countsByYear W20620255032023 @default.
- W2062025503 crossrefType "journal-article" @default.
- W2062025503 hasAuthorship W2062025503A5002064223 @default.
- W2062025503 hasAuthorship W2062025503A5005383720 @default.
- W2062025503 hasAuthorship W2062025503A5015793061 @default.
- W2062025503 hasAuthorship W2062025503A5042649357 @default.
- W2062025503 hasAuthorship W2062025503A5056821715 @default.
- W2062025503 hasAuthorship W2062025503A5068113965 @default.
- W2062025503 hasAuthorship W2062025503A5083354719 @default.
- W2062025503 hasConcept C104317684 @default.
- W2062025503 hasConcept C111599444 @default.
- W2062025503 hasConcept C123012128 @default.
- W2062025503 hasConcept C134018914 @default.
- W2062025503 hasConcept C1491633281 @default.
- W2062025503 hasConcept C15152581 @default.
- W2062025503 hasConcept C153911025 @default.
- W2062025503 hasConcept C202751555 @default.
- W2062025503 hasConcept C2776954459 @default.
- W2062025503 hasConcept C2776992346 @default.
- W2062025503 hasConcept C2777411675 @default.
- W2062025503 hasConcept C2777834122 @default.
- W2062025503 hasConcept C2779395532 @default.
- W2062025503 hasConcept C2780394083 @default.
- W2062025503 hasConcept C2992686903 @default.
- W2062025503 hasConcept C32470452 @default.
- W2062025503 hasConcept C40767141 @default.
- W2062025503 hasConcept C502942594 @default.
- W2062025503 hasConcept C55493867 @default.
- W2062025503 hasConcept C86803240 @default.
- W2062025503 hasConcept C95444343 @default.
- W2062025503 hasConceptScore W2062025503C104317684 @default.
- W2062025503 hasConceptScore W2062025503C111599444 @default.
- W2062025503 hasConceptScore W2062025503C123012128 @default.
- W2062025503 hasConceptScore W2062025503C134018914 @default.
- W2062025503 hasConceptScore W2062025503C1491633281 @default.
- W2062025503 hasConceptScore W2062025503C15152581 @default.
- W2062025503 hasConceptScore W2062025503C153911025 @default.
- W2062025503 hasConceptScore W2062025503C202751555 @default.
- W2062025503 hasConceptScore W2062025503C2776954459 @default.
- W2062025503 hasConceptScore W2062025503C2776992346 @default.
- W2062025503 hasConceptScore W2062025503C2777411675 @default.
- W2062025503 hasConceptScore W2062025503C2777834122 @default.
- W2062025503 hasConceptScore W2062025503C2779395532 @default.
- W2062025503 hasConceptScore W2062025503C2780394083 @default.
- W2062025503 hasConceptScore W2062025503C2992686903 @default.
- W2062025503 hasConceptScore W2062025503C32470452 @default.
- W2062025503 hasConceptScore W2062025503C40767141 @default.
- W2062025503 hasConceptScore W2062025503C502942594 @default.
- W2062025503 hasConceptScore W2062025503C55493867 @default.
- W2062025503 hasConceptScore W2062025503C86803240 @default.
- W2062025503 hasConceptScore W2062025503C95444343 @default.
- W2062025503 hasLocation W20620255031 @default.
- W2062025503 hasOpenAccess W2062025503 @default.
- W2062025503 hasPrimaryLocation W20620255031 @default.
- W2062025503 hasRelatedWork W2011293012 @default.
- W2062025503 hasRelatedWork W2071332814 @default.
- W2062025503 hasRelatedWork W2096590676 @default.
- W2062025503 hasRelatedWork W2366015183 @default.
- W2062025503 hasRelatedWork W2383745058 @default.
- W2062025503 hasRelatedWork W2384929028 @default.
- W2062025503 hasRelatedWork W2410005479 @default.
- W2062025503 hasRelatedWork W2966233672 @default.
- W2062025503 hasRelatedWork W3024900260 @default.
- W2062025503 hasRelatedWork W4212764400 @default.
- W2062025503 hasVolume "10" @default.
- W2062025503 isParatext "false" @default.
- W2062025503 isRetracted "false" @default.
- W2062025503 magId "2062025503" @default.
- W2062025503 workType "article" @default.