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- W2062084414 abstract "The isolation of both specific and nonspecific binding proteins on affinity matrices bearing bioactive compounds hinders the identification of drug cellular targets. Although solid-phase elution and competition methods conventionally are used to distinguish between specific and nonspecific receptor–ligand interactions, these approaches often are severely restricted by low ligand solubility and/or slow kinetic dissociation. This article describes an alternative and versatile method, termed serial affinity chromatography, to identify ligand receptors using affinity resins." @default.
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- W2062084414 date "2006-05-01" @default.
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- W2062084414 title "A versatile method of identifying specific binding proteins on affinity resins" @default.
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- W2062084414 doi "https://doi.org/10.1016/j.ab.2006.02.008" @default.
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