FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2062114510> ?p ?o ?g. }

• W2062114510 endingPage "1418" @default.
• W2062114510 startingPage "1397" @default.
• W2062114510 abstract "West Nile virus (WNV) causes potentially fatal neuroinvasive disease and persists at endemic levels in many parts of the world. Despite advances in our understanding of WNV pathogenesis, there remains a significant need for a human vaccine. The domain III (DIII) region of the WNV envelope protein contains epitopes that are the target of neutralizing antibodies. We have constructed a chimeric fusion of the non-toxic cholera toxin (CT) CTA2/B domains to DIII for investigation as a novel mucosally-delivered WNV vaccine. Purification and assembly of the chimera, as well as receptor-binding and antigen delivery, were verified by western blot, GM1 ELISA and confocal microscopy. Groups of BALB/c mice were immunized intranasally with DIII-CTA2/B, DIII, DIII mixed with CTA2/B, or CTA2/B control, and boosted at 10 days. Analysis of serum IgG after 14 and 45 days revealed that mucosal immunization with DIII-CTA2/B induced significant DIII-specific humoral immunity and drove isotype switching to IgG2a. The DIII-CTA2/B chimera also induced antigen-specific IgM and IgA responses. Bactericidal assays indicate that the DIII-CTA2/B immunized mice produced DIII-specific antibodies that can trigger complement-mediated killing. A dose escalation resulted in increased DIII-specific serum IgG titers on day 45. DIII antigen alone, in the absence of adjuvant, also induced significant systemic responses after intranasal delivery. Our results indicate that the DIII-CTA2/B chimera is immunogenic after intranasal delivery and merits further investigation as a novel WNV vaccine candidate." @default.
• W2062114510 created "2016-06-24" @default.
• W2062114510 creator A5000154039 @default.
• W2062114510 creator A5014866680 @default.
• W2062114510 creator A5057374638 @default.
• W2062114510 creator A5076214468 @default.
• W2062114510 creator A5088904728 @default.
• W2062114510 date "2014-04-22" @default.
• W2062114510 modified "2023-09-27" @default.
• W2062114510 title "Immunogenicity of a West Nile Virus DIII-Cholera Toxin A2/B Chimera after Intranasal Delivery" @default.
• W2062114510 cites W1486323853 @default.
• W2062114510 cites W1490536807 @default.
• W2062114510 cites W1549346812 @default.
• W2062114510 cites W1559720000 @default.
• W2062114510 cites W1613230534 @default.
• W2062114510 cites W1654814305 @default.
• W2062114510 cites W1731649860 @default.
• W2062114510 cites W1755753723 @default.
• W2062114510 cites W1785240960 @default.
• W2062114510 cites W1893064134 @default.
• W2062114510 cites W1954541019 @default.
• W2062114510 cites W1964350435 @default.
• W2062114510 cites W1970136118 @default.
• W2062114510 cites W1975010250 @default.
• W2062114510 cites W1976862305 @default.
• W2062114510 cites W1978453221 @default.
• W2062114510 cites W1978512053 @default.
• W2062114510 cites W1979967313 @default.
• W2062114510 cites W1981732883 @default.
• W2062114510 cites W1982312545 @default.
• W2062114510 cites W1982666738 @default.
• W2062114510 cites W1982954204 @default.
• W2062114510 cites W1985799231 @default.
• W2062114510 cites W1990516872 @default.
• W2062114510 cites W1993098170 @default.
• W2062114510 cites W1995064366 @default.
• W2062114510 cites W2002392006 @default.
• W2062114510 cites W2006104205 @default.
• W2062114510 cites W2010175133 @default.
• W2062114510 cites W2010237430 @default.
• W2062114510 cites W2010305470 @default.
• W2062114510 cites W2015496680 @default.
• W2062114510 cites W2025494340 @default.
• W2062114510 cites W2030949148 @default.
• W2062114510 cites W2033218860 @default.
• W2062114510 cites W2033333432 @default.
• W2062114510 cites W2035562832 @default.
• W2062114510 cites W2037544387 @default.
• W2062114510 cites W2039542138 @default.
• W2062114510 cites W2039841791 @default.
• W2062114510 cites W2043384253 @default.
• W2062114510 cites W2046870976 @default.
• W2062114510 cites W2053845885 @default.
• W2062114510 cites W2055972388 @default.
• W2062114510 cites W2060289140 @default.
• W2062114510 cites W2066108558 @default.
• W2062114510 cites W2066391534 @default.
• W2062114510 cites W2067920276 @default.
• W2062114510 cites W2076294121 @default.
• W2062114510 cites W2078579838 @default.
• W2062114510 cites W2084993024 @default.
• W2062114510 cites W2087606023 @default.
• W2062114510 cites W2095048886 @default.
• W2062114510 cites W2100124746 @default.
• W2062114510 cites W2101056467 @default.
• W2062114510 cites W2101612832 @default.
• W2062114510 cites W2104957765 @default.
• W2062114510 cites W2105092493 @default.
• W2062114510 cites W2105512671 @default.
• W2062114510 cites W2106056357 @default.
• W2062114510 cites W2106226571 @default.
• W2062114510 cites W2108838669 @default.
• W2062114510 cites W2109403620 @default.
• W2062114510 cites W2111997097 @default.
• W2062114510 cites W2112757713 @default.
• W2062114510 cites W2125660667 @default.
• W2062114510 cites W2128568720 @default.
• W2062114510 cites W2132528654 @default.
• W2062114510 cites W2133080338 @default.
• W2062114510 cites W2133676365 @default.
• W2062114510 cites W2139340707 @default.
• W2062114510 cites W2140630012 @default.
• W2062114510 cites W2144552573 @default.
• W2062114510 cites W2146109512 @default.
• W2062114510 cites W2147008913 @default.
• W2062114510 cites W2148235686 @default.
• W2062114510 cites W2151479901 @default.
• W2062114510 cites W2156782507 @default.
• W2062114510 cites W2157852790 @default.
• W2062114510 cites W2158485209 @default.
• W2062114510 cites W2160741006 @default.
• W2062114510 cites W2161616416 @default.
• W2062114510 cites W2163696227 @default.
• W2062114510 cites W2165570312 @default.
• W2062114510 cites W2167192302 @default.
• W2062114510 cites W2168503862 @default.
• W2062114510 cites W2170694569 @default.
• W2062114510 cites W2170812841 @default.

• next