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• W2062114976 abstract "Tumor-associated trypsin inhibitor (TATI) is mainly proposed as a tumor marker for ovarian cancer. However, recent discoveries of TATI in cancer and inflammatory diseases show that assay of TATI in biological samples is of increasing interest. We validated a previously described TR-IFMA and a newly developed dual-monoclonal sandwich ELISA for TATI. These methods were compared with a commercial radioimmunoassay (RIA). We studied preanalytical factors affecting serum TATI concentrations and established age- and gender specific reference intervals using serum samples from 195 healthy volunteers. The assay range and precision were 0.8–45 μg/L and < 9.1% for the ELISA, and 0.13 μg/L–150 μg/L and < 12.5% for the TR-IFMA, respectively. Both assays correlated well with a RIA. Type of blood sample and nutritional status were not critical and TATI was stable in serum samples when stored at + 4 °C and − 20 °C for 4 weeks and at − 80 °C for 8 weeks. The 95% reference limits for serum TATI in adults were 5.2–15.3 μg/L in the age group 18–70 years and 7.5–21.3 μg/L in the age group > 70 years. Significantly higher concentrations of serum TATI were observed in elderly women and in men. Both ELISA and TR-IFMA technologies can be employed to develop sensitive and robust immunoassays for TATI using monoclonal antibodies." @default.
• W2062114976 created "2016-06-24" @default.
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• W2062114976 date "2012-08-01" @default.
• W2062114976 modified "2023-10-18" @default.
• W2062114976 title "Validation and comparison of tumor-associated trypsin inhibitor (TATI) immunoassays" @default.
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• W2062114976 doi "https://doi.org/10.1016/j.cca.2012.04.001" @default.
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