FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2062121465> ?p ?o ?g. }

• W2062121465 endingPage "890" @default.
• W2062121465 startingPage "884" @default.
• W2062121465 abstract "In Brief Study Design. Rat lumbar discs comprising nucleus pulposus, annulus fibrosus, and cartilaginous endplates were cultured for 1 week in a specialized media containing either TGF-β1 or TGF-β3. Role of TGF-β isoforms on cell function was evaluated. Objective. To develop an in vitro organ culture of rat intervertebral disc and evaluate effects of TGF-β3 on disc cell function. Summary of Background Data. An in vitro model system is of considerable value in understanding the cell biology of the intervertebral disc. Development of a useful organ culture model would enhance understanding of disc function in health and disease. Materials and Methods. Rat lumbar intervertebral discs were maintained in organ culture in media supplemented with TGF-β3 or TGF-β1 for 1 week. Tissue morphology was studied using routine histologic, histochemical and immunohistochemical techniques. Cell function was assessed by gene expression, sulfate incorporation, and Western blot analysis. Results. After 1 week in culture with TGF-β3 and TGF-β1, the gross morphology and tissue architecture of the disc were preserved. TUNEL analysis indicated that there was no evidence of cell death in the nucleus pulposus or the anulus fibrosus. The level of Alcian blue staining in the nucleus pulposus was similar to that of the freshly isolated disc. However, when compared with TGF-β1, TGF-β3 elevated the expression of critical matrix genes, enhanced [35S] incorporation into proteoglycans, preserved the expression of TGF-β receptors, and decreased aggrecan turnover. There was also increased activation (phosphorylation) of ERK, a critical signaling protein. Moreover, inhibition of ERK activity, in the presence TGF-β3, resulted in suppression of collagen Type II, aggrecan, TGF-β-RI, TGF-β-RII and TGF-β-RIII mRNA expression. Conclusions. TGF-β3 maintains the phenotype of disc cells in organ culture. It exerts this effect, in part, by elevating the levels of activated ERK1/2, which in turn regulates the expression of TGF-β-RI and TGF-β-RII. Development of a useful in vitro culture model would enhance the understanding of disc cell function in health and disease. In the present study, we demonstrate that TGF-β3 enhances the survival and viability of cells of the intervertebral disc in organ culture. It exerts this effect, in part, by elevating the levels of activated ERK1/2, which regulates the expression of TGF-β-RI and TGF-β-RII." @default.
• W2062121465 created "2016-06-24" @default.
• W2062121465 creator A5009142068 @default.
• W2062121465 creator A5011224388 @default.
• W2062121465 creator A5012718663 @default.
• W2062121465 creator A5019521528 @default.
• W2062121465 creator A5025963070 @default.
• W2062121465 creator A5030209609 @default.
• W2062121465 creator A5035737240 @default.
• W2062121465 creator A5046825258 @default.
• W2062121465 date "2006-04-01" @default.
• W2062121465 modified "2023-10-09" @default.
• W2062121465 title "Toward an Optimum System for Intervertebral Disc Organ Culture" @default.
• W2062121465 cites W1503882644 @default.
• W2062121465 cites W1965282660 @default.
• W2062121465 cites W1973847681 @default.
• W2062121465 cites W1994738588 @default.
• W2062121465 cites W2001093897 @default.
• W2062121465 cites W2002559995 @default.
• W2062121465 cites W2008236596 @default.
• W2062121465 cites W2013515157 @default.
• W2062121465 cites W2013958991 @default.
• W2062121465 cites W2034035558 @default.
• W2062121465 cites W2041172831 @default.
• W2062121465 cites W2048524426 @default.
• W2062121465 cites W2059808378 @default.
• W2062121465 cites W2066089068 @default.
• W2062121465 cites W2066687881 @default.
• W2062121465 cites W2069487258 @default.
• W2062121465 cites W2079393806 @default.
• W2062121465 cites W2138959887 @default.
• W2062121465 cites W2142579272 @default.
• W2062121465 cites W2154568166 @default.
• W2062121465 cites W2154601080 @default.
• W2062121465 cites W2406721410 @default.
• W2062121465 cites W2474088718 @default.
• W2062121465 cites W4296588288 @default.
• W2062121465 doi "https://doi.org/10.1097/01.brs.0000209335.57767.b5" @default.
• W2062121465 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16622376" @default.
• W2062121465 hasPublicationYear "2006" @default.
• W2062121465 type Work @default.
• W2062121465 sameAs 2062121465 @default.
• W2062121465 citedByCount "98" @default.
• W2062121465 countsByYear W20621214652012 @default.
• W2062121465 countsByYear W20621214652013 @default.
• W2062121465 countsByYear W20621214652014 @default.
• W2062121465 countsByYear W20621214652015 @default.
• W2062121465 countsByYear W20621214652016 @default.
• W2062121465 countsByYear W20621214652017 @default.
• W2062121465 countsByYear W20621214652018 @default.
• W2062121465 countsByYear W20621214652019 @default.
• W2062121465 countsByYear W20621214652020 @default.
• W2062121465 countsByYear W20621214652021 @default.
• W2062121465 countsByYear W20621214652022 @default.
• W2062121465 countsByYear W20621214652023 @default.
• W2062121465 crossrefType "journal-article" @default.
• W2062121465 hasAuthorship W2062121465A5009142068 @default.
• W2062121465 hasAuthorship W2062121465A5011224388 @default.
• W2062121465 hasAuthorship W2062121465A5012718663 @default.
• W2062121465 hasAuthorship W2062121465A5019521528 @default.
• W2062121465 hasAuthorship W2062121465A5025963070 @default.
• W2062121465 hasAuthorship W2062121465A5030209609 @default.
• W2062121465 hasAuthorship W2062121465A5035737240 @default.
• W2062121465 hasAuthorship W2062121465A5046825258 @default.
• W2062121465 hasConcept C105702510 @default.
• W2062121465 hasConcept C118131993 @default.
• W2062121465 hasConcept C126322002 @default.
• W2062121465 hasConcept C142724271 @default.
• W2062121465 hasConcept C197534660 @default.
• W2062121465 hasConcept C202751555 @default.
• W2062121465 hasConcept C204787440 @default.
• W2062121465 hasConcept C2776164576 @default.
• W2062121465 hasConcept C2777755357 @default.
• W2062121465 hasConcept C2780723820 @default.
• W2062121465 hasConcept C28483552 @default.
• W2062121465 hasConcept C3018239782 @default.
• W2062121465 hasConcept C3020332539 @default.
• W2062121465 hasConcept C44575665 @default.
• W2062121465 hasConcept C54355233 @default.
• W2062121465 hasConcept C55493867 @default.
• W2062121465 hasConcept C71924100 @default.
• W2062121465 hasConcept C81885089 @default.
• W2062121465 hasConcept C86803240 @default.
• W2062121465 hasConcept C95444343 @default.
• W2062121465 hasConceptScore W2062121465C105702510 @default.
• W2062121465 hasConceptScore W2062121465C118131993 @default.
• W2062121465 hasConceptScore W2062121465C126322002 @default.
• W2062121465 hasConceptScore W2062121465C142724271 @default.
• W2062121465 hasConceptScore W2062121465C197534660 @default.
• W2062121465 hasConceptScore W2062121465C202751555 @default.
• W2062121465 hasConceptScore W2062121465C204787440 @default.
• W2062121465 hasConceptScore W2062121465C2776164576 @default.
• W2062121465 hasConceptScore W2062121465C2777755357 @default.
• W2062121465 hasConceptScore W2062121465C2780723820 @default.
• W2062121465 hasConceptScore W2062121465C28483552 @default.
• W2062121465 hasConceptScore W2062121465C3018239782 @default.
• W2062121465 hasConceptScore W2062121465C3020332539 @default.
• W2062121465 hasConceptScore W2062121465C44575665 @default.

• next