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- W2062207310 abstract "We label ribonuclease S with a 3 nm Au nanoparticle (NP) by utilizing its two-piece structure. One portion, S-peptide, is mutated with a unique NP attachment site. NP-peptide self-assembles with the other portion, S-protein, to form an active enzyme. NP mobility decreases with peptide labeling and S-protein association. Surface plasmon shifts support conjugation. Higher S-peptide coverages on the NP surface reduce nonspecific adsorption, while sterically hindering assembly of RNaseS. Thiols displace nonspecific adsorption, maximizing site-specific labeling." @default.
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- W2062207310 date "2005-02-18" @default.
- W2062207310 modified "2023-09-30" @default.
- W2062207310 title "Labeling Ribonuclease S with a 3 nm Au Nanoparticle by Two-Step Assembly" @default.
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- W2062207310 doi "https://doi.org/10.1021/nl0479031" @default.
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