FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2062207918> ?p ?o ?g. }

• W2062207918 endingPage "e96893" @default.
• W2062207918 startingPage "e96893" @default.
• W2062207918 abstract "Peptidases are ubiquitous enzymes involved in diverse biological processes. Fragments from bioactive peptides have been found in skin secretions from frogs, and their presence suggests processing by peptidases. Thus, the aim of this work was to characterize the peptidase activity present in the skin secretion of Leptodactylus labyrinthicus. Zymography revealed the presence of three bands of gelatinase activity of approximately 60 kDa, 66 kDa, and 80 kDa, which the first two were calcium-dependent. These three bands were inhibited either by ethylenediaminetetraacetic acid (EDTA) and phenathroline; thus, they were characterized as metallopeptidases. Furthermore, the proteolytic enzymes identified were active only at pH 6.0–10.0, and their activity increased in the presence of CHAPS or NaCl. Experiments with fluorogenic substrates incubated with skin secretions identified aminopeptidase activity, with cleavage after leucine, proline, and alanine residues. This activity was directly proportional to the protein concentration, and it was inhibited in the presence of metallo and serine peptidase inhibitors. Besides, the optimal pH for substrate cleavage was determined to be 7.0–8.0. The results of the in gel activity assay showed that all substrates were hydrolyzed by a 45 kDa peptidase. Gly-Pro-AMC was also cleaved by a peptidase greater than 97 kDa. The data suggest the presence of dipeptidyl peptidases (DPPs) and metallopeptidases; however, further research is necessary. In conclusion, our work will help to elucidate the implication of these enzymatic activities in the processing of the bioactive peptides present in frog venom, expanding the knowledge of amphibian biology." @default.
• W2062207918 created "2016-06-24" @default.
• W2062207918 creator A5013615191 @default.
• W2062207918 creator A5024929522 @default.
• W2062207918 creator A5042649462 @default.
• W2062207918 creator A5058201358 @default.
• W2062207918 creator A5068603567 @default.
• W2062207918 creator A5083256606 @default.
• W2062207918 date "2014-06-06" @default.
• W2062207918 modified "2023-09-23" @default.
• W2062207918 title "The Crude Skin Secretion of the Pepper Frog Leptodactylus labyrinthicus Is Rich in Metallo and Serine Peptidases" @default.
• W2062207918 cites W1484296597 @default.
• W2062207918 cites W1526178345 @default.
• W2062207918 cites W1965395493 @default.
• W2062207918 cites W1966733674 @default.
• W2062207918 cites W1967845976 @default.
• W2062207918 cites W1969432608 @default.
• W2062207918 cites W1969880960 @default.
• W2062207918 cites W1970662644 @default.
• W2062207918 cites W1973505268 @default.
• W2062207918 cites W1983548824 @default.
• W2062207918 cites W1984007337 @default.
• W2062207918 cites W1986350809 @default.
• W2062207918 cites W1986725457 @default.
• W2062207918 cites W1987699359 @default.
• W2062207918 cites W1988228153 @default.
• W2062207918 cites W2004038025 @default.
• W2062207918 cites W2006100483 @default.
• W2062207918 cites W2006227848 @default.
• W2062207918 cites W2008961537 @default.
• W2062207918 cites W2009393104 @default.
• W2062207918 cites W2012729708 @default.
• W2062207918 cites W2013896356 @default.
• W2062207918 cites W2014319618 @default.
• W2062207918 cites W2019163148 @default.
• W2062207918 cites W2020790969 @default.
• W2062207918 cites W2024239571 @default.
• W2062207918 cites W2024535827 @default.
• W2062207918 cites W2032513705 @default.
• W2062207918 cites W2033754198 @default.
• W2062207918 cites W2035316083 @default.
• W2062207918 cites W2039692969 @default.
• W2062207918 cites W2039805448 @default.
• W2062207918 cites W2044853699 @default.
• W2062207918 cites W2045225257 @default.
• W2062207918 cites W2045727281 @default.
• W2062207918 cites W2045846497 @default.
• W2062207918 cites W2057573335 @default.
• W2062207918 cites W2060328315 @default.
• W2062207918 cites W2060486005 @default.
• W2062207918 cites W2067368663 @default.
• W2062207918 cites W2068062399 @default.
• W2062207918 cites W2071601558 @default.
• W2062207918 cites W2075878614 @default.
• W2062207918 cites W2076144702 @default.
• W2062207918 cites W2076245642 @default.
• W2062207918 cites W2077043569 @default.
• W2062207918 cites W2078845279 @default.
• W2062207918 cites W2084145226 @default.
• W2062207918 cites W2085507541 @default.
• W2062207918 cites W2087394105 @default.
• W2062207918 cites W2095076390 @default.
• W2062207918 cites W2119979229 @default.
• W2062207918 cites W2143483161 @default.
• W2062207918 cites W2156069524 @default.
• W2062207918 cites W2165434282 @default.
• W2062207918 cites W2168519444 @default.
• W2062207918 cites W2246824184 @default.
• W2062207918 cites W4246096964 @default.
• W2062207918 cites W4247333866 @default.
• W2062207918 cites W4252236782 @default.
• W2062207918 cites W4382128165 @default.
• W2062207918 cites W45139103 @default.
• W2062207918 doi "https://doi.org/10.1371/journal.pone.0096893" @default.
• W2062207918 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4048173" @default.
• W2062207918 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24906116" @default.
• W2062207918 hasPublicationYear "2014" @default.
• W2062207918 type Work @default.
• W2062207918 sameAs 2062207918 @default.
• W2062207918 citedByCount "13" @default.
• W2062207918 countsByYear W20622079182015 @default.
• W2062207918 countsByYear W20622079182016 @default.
• W2062207918 countsByYear W20622079182017 @default.
• W2062207918 countsByYear W20622079182018 @default.
• W2062207918 countsByYear W20622079182019 @default.
• W2062207918 countsByYear W20622079182020 @default.
• W2062207918 countsByYear W20622079182021 @default.
• W2062207918 crossrefType "journal-article" @default.
• W2062207918 hasAuthorship W2062207918A5013615191 @default.
• W2062207918 hasAuthorship W2062207918A5024929522 @default.
• W2062207918 hasAuthorship W2062207918A5042649462 @default.
• W2062207918 hasAuthorship W2062207918A5058201358 @default.
• W2062207918 hasAuthorship W2062207918A5068603567 @default.
• W2062207918 hasAuthorship W2062207918A5083256606 @default.
• W2062207918 hasBestOaLocation W20622079181 @default.
• W2062207918 hasConcept C177430391 @default.
• W2062207918 hasConcept C181199279 @default.
• W2062207918 hasConcept C182220744 @default.

• next