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- W2062244473 abstract "The testicular regions of male mice were exposed to x-ray doses ranging from 0 to 400 rads. Forty days after exposure the mice were killed and the testes and cauda epididymal sperm removed surgically. Flow cytometric measurements of acridine orange stained testicular samples indicated a repopulation of testicular cell types following x-ray killing of stem cells. Cauda epididymal sperm were analyzed by the sperm chromatin structure assay (SCSA), a flow cytometric measurement of the susceptibility of the sperm nuclear DNA to in situ acid denaturation. The SCSA detected increased susceptibility to DNA denaturation in situ after 12.5 rods of x-ray exposure, with significant increases following 25 rads. Abnormal sperm head morphology was not significantly increased until the testes were exposed to 60 rads of x-rays. These data suggest that the SCSA is currently the most sensitive, non-invasive method of detecting x-ray damage to testicular stem spermatogonia. © 1995 Wiley-Liss, Inc." @default.
- W2062244473 created "2016-06-24" @default.
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- W2062244473 date "1995-01-01" @default.
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- W2062244473 title "Effects of X-irradiation on mouse testicular cells and sperm chromatin structure" @default.
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- W2062244473 doi "https://doi.org/10.1002/em.2850250105" @default.
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