Matches in SemOpenAlex for { <https://semopenalex.org/work/W2062245356> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2062245356 endingPage "38" @default.
- W2062245356 startingPage "38" @default.
- W2062245356 abstract "Metabolic transformations of retinoids within the retinal photoreceptor and retinal pigment epithelial (RPE) cells are responsible for the production of the visual chromophore, 11-cis-retinal, which is essential for vision. Within RPE cells, all-trans-retinol (vitamin A), a product of photoisomerised and reduced 11-cis-retinal, is esterified with fatty acids in a reaction catalyzed by lecithin:retinol acyl transferase (LRAT). While a number of retinoid metabolites are generated during regeneration of 11-cis-retinal, only retinol and retinyl esters show a weak intrinsic fluorescence (excitation l ex at ∼320 nm). Two-photon excitation laser scanning microscopy was applied as a noninvasive imaging technique to investigate the light-dependent distribution of fluorescent all-trans retinol and all-trans retinyl ester in the dissected mouse eye. Using the intrinsic fluorescence of all-trans-retinyl esters, noninvasive two-photon microscopy in combination with HPLC analysis revealed previously uncharacterized structures distinct from other cellular organelles, termed the retinyl ester storage particles or retinosomes. These structures form autonomous all-trans-retinyl ester-rich intracellular compartments distinct from other organelles and colocalize with adipose differentiation-related protein (ADRP). As demonstrated by in vivo experiments using wild-type mice, the retinosomes participate in 11-cis-retinal formation. Retinosomes accumulate in Rpe65-/- mice incapable of carrying out the enzymatic isomerization, and correspondingly, are absent in the eyes of Lrat-/- mice deficient in retinyl ester synthesis. These results indicate that retinosomes located close to the RPE lateral plasma membrane are essential components in 11-cis-retinal production." @default.
- W2062245356 created "2016-06-24" @default.
- W2062245356 creator A5042148986 @default.
- W2062245356 date "2010-03-28" @default.
- W2062245356 modified "2023-09-26" @default.
- W2062245356 title "Noninvasive two-photon imaging reveals retinyl ester storage structures in the eye" @default.
- W2062245356 doi "https://doi.org/10.1167/6.13.38" @default.
- W2062245356 hasPublicationYear "2010" @default.
- W2062245356 type Work @default.
- W2062245356 sameAs 2062245356 @default.
- W2062245356 citedByCount "1" @default.
- W2062245356 crossrefType "journal-article" @default.
- W2062245356 hasAuthorship W2062245356A5042148986 @default.
- W2062245356 hasBestOaLocation W20622453561 @default.
- W2062245356 hasConcept C104317684 @default.
- W2062245356 hasConcept C12554922 @default.
- W2062245356 hasConcept C131667965 @default.
- W2062245356 hasConcept C168240541 @default.
- W2062245356 hasConcept C185592680 @default.
- W2062245356 hasConcept C202033177 @default.
- W2062245356 hasConcept C2775954498 @default.
- W2062245356 hasConcept C2776940978 @default.
- W2062245356 hasConcept C2777326149 @default.
- W2062245356 hasConcept C2779573558 @default.
- W2062245356 hasConcept C2780827179 @default.
- W2062245356 hasConcept C2781121885 @default.
- W2062245356 hasConcept C2909445175 @default.
- W2062245356 hasConcept C55493867 @default.
- W2062245356 hasConcept C86803240 @default.
- W2062245356 hasConcept C95444343 @default.
- W2062245356 hasConceptScore W2062245356C104317684 @default.
- W2062245356 hasConceptScore W2062245356C12554922 @default.
- W2062245356 hasConceptScore W2062245356C131667965 @default.
- W2062245356 hasConceptScore W2062245356C168240541 @default.
- W2062245356 hasConceptScore W2062245356C185592680 @default.
- W2062245356 hasConceptScore W2062245356C202033177 @default.
- W2062245356 hasConceptScore W2062245356C2775954498 @default.
- W2062245356 hasConceptScore W2062245356C2776940978 @default.
- W2062245356 hasConceptScore W2062245356C2777326149 @default.
- W2062245356 hasConceptScore W2062245356C2779573558 @default.
- W2062245356 hasConceptScore W2062245356C2780827179 @default.
- W2062245356 hasConceptScore W2062245356C2781121885 @default.
- W2062245356 hasConceptScore W2062245356C2909445175 @default.
- W2062245356 hasConceptScore W2062245356C55493867 @default.
- W2062245356 hasConceptScore W2062245356C86803240 @default.
- W2062245356 hasConceptScore W2062245356C95444343 @default.
- W2062245356 hasIssue "13" @default.
- W2062245356 hasLocation W20622453561 @default.
- W2062245356 hasOpenAccess W2062245356 @default.
- W2062245356 hasPrimaryLocation W20622453561 @default.
- W2062245356 hasRelatedWork W1823874834 @default.
- W2062245356 hasRelatedWork W1975358267 @default.
- W2062245356 hasRelatedWork W1993132210 @default.
- W2062245356 hasRelatedWork W2001778500 @default.
- W2062245356 hasRelatedWork W2007416260 @default.
- W2062245356 hasRelatedWork W2014710880 @default.
- W2062245356 hasRelatedWork W2016100843 @default.
- W2062245356 hasRelatedWork W2026416813 @default.
- W2062245356 hasRelatedWork W2068593589 @default.
- W2062245356 hasRelatedWork W2090539847 @default.
- W2062245356 hasVolume "6" @default.
- W2062245356 isParatext "false" @default.
- W2062245356 isRetracted "false" @default.
- W2062245356 magId "2062245356" @default.
- W2062245356 workType "article" @default.