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• W2062280810 abstract "Cell sublines were derived from Ni-induced rat rhabdomyosarcoma which differed in their degree of tumorigenicity. We compared the expression of protein kinase C isoforms alpha, beta, gamma and epsilon in three sublines developing tumors in syngeneic rats (Sy+) and in two sublines devoid of tumorigenicity in these animals (Sy-), with that of normal skeletal muscle. Northern and Western blotting experiments showed that PKC alpha was dramatically overexpressed in Sy- cells whereas it was underexpressed in Sy+ cells. Southern blot analysis provided evidence for a 3-fold increase of PKC alpha-related DNA fragments in Sy- cells. Steady-state levels of the PKC epsilon-related transcript were also markedly decreased in Sy+ cells. However, the expression of PKC beta-related RNA was increased in these cells. Our data support the concept that the differential expression of the PKC isoforms may play a critical role in determining the neoplastic phenotype." @default.
• W2062280810 created "2016-06-24" @default.
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• W2062280810 date "1992-05-25" @default.
• W2062280810 modified "2023-10-18" @default.
• W2062280810 title "Tumorigenicity-associated expression of protein kinase C isoforms in rhabdomyosarcoma-derived cells" @default.
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• W2062280810 doi "https://doi.org/10.1016/0014-5793(92)80467-u" @default.
• W2062280810 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1592108" @default.
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