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- W2062297586 abstract "Abstract Since the first electrochemical reduction step of NAD÷ and the electrochemical oxidation step of NADH lie respectively at —0.69 and 0.5–0.9 V (N.H.E.). no direct measure of the formal potential of NAD÷-NADH system may be attained potentiometrically. We examine the significance of the previous potentiometric studies realized in the presence of a mediator and an enzyme. A reliable zer-current potential of NAD÷ and NADH solution is only obtained when small amounts of benzyl-viologen (BV 2÷ ) and xanthine oxidase (XO) are added. The various electrochemical reduction steps of 10 to 1000 μ M BV 2÷ aqueous solutions are studied at pH 9.8 on dropping mercury and rotating platinum disk electrodes. The formal potential of the BV 2÷ -BV − system, equal to —0.360 V (N.H.E.), may be measured on a platinum electrode, while on a mercury electrode a strong adsorption interferes and gives a polarographic prewave. When XO is present, the addition of NADH decreases the BV 2÷ reduction waves: an anodic wave corresponding to BV 2÷ oxidation appears at the same potential as the first BV 2÷ reduction wave. The zero-current potential measured in NAD÷, NADH, BV 2÷ , BV 2÷ and XO solutions is actually fixed by the BV 2÷ -BV÷ system, which equilibrates through chemical oxido-reduction reactions with the NAD÷-NADH system." @default.
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- W2062297586 date "1976-01-01" @default.
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- W2062297586 title "Significance of the Zero-Current Potential of the NAD+-NADH system" @default.
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- W2062297586 doi "https://doi.org/10.1016/0302-4598(76)80040-2" @default.
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