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• W2062316703 abstract "We report here a transposon-based strategy to generate Streptomyces globisporus 1912 mutants with improved landomycin E production. The modified minitransposon with strong, outward-oriented promoters for the overexpression of downstream-situated genes has been applied for mutant library generation. Approximately 2500 mutants of S. globisporus 1912 were analyzed for landomycin E production, leading to the identification of several overproducers. Subcloning and sequencing of the sites of integration showed that some of the inactivated genes encode proteins with a similarity to known bacterial regulators such as TetR and LuxR families. One of the regulators (GntR type) has shown the strongest influence on the landomycin E production. Its ortholog (encoded by sco3269) in Streptomyces coelicolor was characterized in greater detail and showed similar effects on actinorhodin production and morphological differentiation." @default.
• W2062316703 created "2016-06-24" @default.
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• W2062316703 date "2013-04-24" @default.
• W2062316703 modified "2023-10-03" @default.
• W2062316703 title "A transposon-based strategy to identify the regulatory gene network responsible for landomycin E biosynthesis" @default.
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• W2062316703 doi "https://doi.org/10.1111/1574-6968.12117" @default.
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