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• W206232011 abstract "Research Article1 February 1983free access Specific DNA binding of the cAMP receptor protein within the lac operon stabilizes double-stranded DNA in the presence of cAMP. B. Unger B. Unger Division of Molecular Pharmacology, National Institute for Medical Research, London, UK. Search for more papers by this author G.M. Clore G.M. Clore Division of Molecular Pharmacology, National Institute for Medical Research, London, UK. Search for more papers by this author A.M. Gronenborn A.M. Gronenborn Division of Molecular Pharmacology, National Institute for Medical Research, London, UK. Search for more papers by this author W. Hillen W. Hillen Division of Molecular Pharmacology, National Institute for Medical Research, London, UK. Search for more papers by this author B. Unger B. Unger Division of Molecular Pharmacology, National Institute for Medical Research, London, UK. Search for more papers by this author G.M. Clore G.M. Clore Division of Molecular Pharmacology, National Institute for Medical Research, London, UK. Search for more papers by this author A.M. Gronenborn A.M. Gronenborn Division of Molecular Pharmacology, National Institute for Medical Research, London, UK. Search for more papers by this author W. Hillen W. Hillen Division of Molecular Pharmacology, National Institute for Medical Research, London, UK. Search for more papers by this author Author Information B. Unger1, G.M. Clore1, A.M. Gronenborn1 and W. Hillen1 1Division of Molecular Pharmacology, National Institute for Medical Research, London, UK. The EMBO Journal (1983)2:289-293https://doi.org/10.1002/j.1460-2075.1983.tb01419.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The effects of varying amounts of cAMP receptor protein (CRP) in the presence and absence of cAMP on the melting and differential melting curves of a 301-bp fragment containing the lac control region in 5 mM Na+ have been investigated. The native 301-bp fragment consists of three cooperatively melting thermalites. At 5 mM Na+, thermalite I (155 bp) has a Tm of 66.4 degrees C and the melting transitions of thermalites II (81 bp) and III (65 bp) are superimposed with a Tm of 61.9 degrees C. The specific DNA target site for CRP and the lac promotor are located within thermalite II. CRP alone exerts no specific effects on the melting of the 301-bp fragment, non-specific DNA binding of CRP resulting in a progressive stabilization of the double-stranded DNA by increasing the number of base pairs melting at a higher Tm in a non-cooperative transition. The cAMP-CRP complex, however, exerts a specific effect with a region of approximately 36 bp, comprising the specific CRP binding site and a neighbouring region of DNA, being stabilized. The appearance of this new cooperatively melting region, known as thermalite IV, is associated with a corresponding decrease in the area of thermalites II/III. The Tm of thermalite IV is 64.4 degrees C, 2.5 degrees C higher than that of thermalites II/III. With two or more cAMP-CRP complexes bound per 301-bp fragment, the stabilization also affects the remaining 110 bp now making up thermalites II/III whose Tm is increased by 1 degrees C to 62.9 degrees C. The implications of these findings for various models of the mode of action of the cAMP-CRP complex are discussed. Next ArticlePrevious Article Volume 2Issue 21 February 1983In this issue RelatedDetailsLoading ..." @default.
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• W206232011 title "Specific DNA binding of the cAMP receptor protein within the lac operon stabilizes double-stranded DNA in the presence of cAMP." @default.
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