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• W2062333547 abstract "The successful cryopreservation of mammalian embryos is often attributed to the application of theoretical considerations and empirical observations derived from other cellular systems. Two factors were found to be important in the cryopreservation of embryos, namely, the presence of molar concentrations of a protective solute such as dimethylsulfoxide (Me2SO) or glycerol, and the appropriate control of the rates of cooling and warming (15, 44, 48). Cryoprotective solutes of low molecular weight are thought to prevent the potentially deleterious exposure of cells to elevated concentrations of electrolytes by their colligative action in reducing the quantity of ice formed at any subzero temperature (17, 33). Control of the cooling and warming conditions determines the ultimate fate of intracellular water during the cryopreservation process (24). If the rate of cooling is sufficiently low, cytoplasmic water will flow out of the cells and freeze extracellularly. However, if cooling is too rapid, the cytoplasm will not have sufficient time to dehydrate. As a result, the cytoplasm will supercool and eventually freeze. The formation of intracellular ice is thought to lead to injury (26). Some studies, however, suggest that under" @default.
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• W2062333547 date "1984-02-01" @default.
• W2062333547 modified "2023-10-18" @default.
• W2062333547 title "Analysis of slow-warming injury of mouse embryos by cryomicroscopical and physiochemical methods" @default.
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• W2062333547 doi "https://doi.org/10.1016/0011-2240(84)90027-0" @default.
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