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• W2062341643 endingPage "11553" @default.
• W2062341643 startingPage "11540" @default.
• W2062341643 abstract "In traumatic brain injury (TBI), neurons surviving the primary insult may succumb through poorly understood secondary mechanisms. In vitro , cortical neurons exposed to stretch injury exhibited enhanced vulnerability to NMDA, apoptotic-like DNA fragmentation, peroxynitrite (PN) formation, and cytoplasmic cytochrome c accumulation. Surprisingly, caspase-3 activity was undetectable by both immunoblotting and fluorogenic activity assays. Therefore, we hypothesized that PN directly inhibits caspases in these neurons. Consistent with this, stretch injury in cultured neurons elicited tyrosine nitration of procaspase-3, but not caspase-9 or Apaf-1, suggesting a direct interaction of PN with caspase-3. In an ex vivo system, PN inhibited the activity of caspase-3, and this inhibition was reversible with the addition of the sulfhydryl reducing agent dithiothreitol, indicating that PN inhibits caspases by cysteinyl oxidation. Moreover, in cultures, the PN donor 3-morpholinosydnonimine (SIN-1) blocked staurosporine-induced caspase-3 activation and its downstream effects including PARP-1 [poly-(ADP-ribose) polymerase-1] cleavage and phosphotidylserine inversion, suggesting that peroxynitrite can inhibit caspase-3-mediated apoptosis. To examine these mechanisms in vivo , rats were exposed to a lateral fluid percussion injury (FPI). FPI caused increased neuronal protein nitration that colocalized with TUNEL staining, indicating that PN was associated with neurodegeneration. Caspase-3 activity was inhibited in brain lysates harvested after FPI and was restored by adding dithiothreitol. Our data show that caspase-mediated apoptosis is inhibited in neurons subjected to stretch in vitro and to TBI in vivo , mostly because of cysteinyl oxidation of caspase-3 by PN. However, this is insufficient to prevent cell death, indicating that the TBI therapy may, at a minimum, require a combination of both anti-apoptotic and anti-oxidant strategies." @default.
• W2062341643 created "2016-06-24" @default.
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• W2062341643 date "2006-11-08" @default.
• W2062341643 modified "2023-10-13" @default.
• W2062341643 title "Inhibition of Caspase-Mediated Apoptosis by Peroxynitrite in Traumatic Brain Injury" @default.
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• W2062341643 cites W1506642042 @default.
• W2062341643 cites W1512743604 @default.
• W2062341643 cites W1520169101 @default.
• W2062341643 cites W1522782595 @default.
• W2062341643 cites W1542912083 @default.
• W2062341643 cites W1568752613 @default.
• W2062341643 cites W1594873093 @default.
• W2062341643 cites W1821918060 @default.
• W2062341643 cites W1829813323 @default.
• W2062341643 cites W1868062960 @default.
• W2062341643 cites W1928214158 @default.
• W2062341643 cites W1940692702 @default.
• W2062341643 cites W1963857988 @default.
• W2062341643 cites W1966971174 @default.
• W2062341643 cites W1967544492 @default.
• W2062341643 cites W1968793573 @default.
• W2062341643 cites W1978679885 @default.
• W2062341643 cites W1981023844 @default.
• W2062341643 cites W1981958496 @default.
• W2062341643 cites W1982785786 @default.
• W2062341643 cites W1983455034 @default.
• W2062341643 cites W1984609402 @default.
• W2062341643 cites W1989528243 @default.
• W2062341643 cites W1990798856 @default.
• W2062341643 cites W1992177920 @default.
• W2062341643 cites W1993128970 @default.
• W2062341643 cites W1995757963 @default.
• W2062341643 cites W1999649376 @default.
• W2062341643 cites W2002631122 @default.
• W2062341643 cites W2010298858 @default.
• W2062341643 cites W2012844571 @default.
• W2062341643 cites W2018057744 @default.
• W2062341643 cites W2021492387 @default.
• W2062341643 cites W2022647670 @default.
• W2062341643 cites W2023462778 @default.
• W2062341643 cites W2024267786 @default.
• W2062341643 cites W2025219416 @default.
• W2062341643 cites W2026364404 @default.
• W2062341643 cites W2028531025 @default.
• W2062341643 cites W2028697168 @default.
• W2062341643 cites W2029068965 @default.
• W2062341643 cites W2032981028 @default.
• W2062341643 cites W2033624750 @default.
• W2062341643 cites W2034117088 @default.
• W2062341643 cites W2034679108 @default.
• W2062341643 cites W2036162274 @default.
• W2062341643 cites W2037279622 @default.
• W2062341643 cites W2038933726 @default.
• W2062341643 cites W2047936141 @default.
• W2062341643 cites W2051600991 @default.
• W2062341643 cites W2051961329 @default.
• W2062341643 cites W2053846841 @default.
• W2062341643 cites W2062445457 @default.
• W2062341643 cites W2063262838 @default.
• W2062341643 cites W2065730275 @default.
• W2062341643 cites W2075729900 @default.
• W2062341643 cites W2076245761 @default.
• W2062341643 cites W2076731990 @default.
• W2062341643 cites W2078579431 @default.
• W2062341643 cites W2093708814 @default.
• W2062341643 cites W2113718307 @default.
• W2062341643 cites W2113999599 @default.
• W2062341643 cites W2118440238 @default.
• W2062341643 cites W2119683782 @default.
• W2062341643 cites W2124748247 @default.
• W2062341643 cites W2130866079 @default.
• W2062341643 cites W2130995732 @default.
• W2062341643 cites W2137832426 @default.
• W2062341643 cites W2147783395 @default.
• W2062341643 cites W2150445605 @default.
• W2062341643 cites W2152102580 @default.
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• W2062341643 doi "https://doi.org/10.1523/jneurosci.3507-06.2006" @default.
• W2062341643 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6674768" @default.
• W2062341643 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17093075" @default.
• W2062341643 hasPublicationYear "2006" @default.
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