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• W2062516474 abstract "A pair of small plasmid vectors have been constructed, which complement chlorate-resistant chlB mutants of Escherichia coli to chlorate reduction under anaerobic conditions. This potentially lethal function can be inactivated by the insertion of DNA fragments into a multiple cloning site. After transformation of competent mutant cells with a ligation mixture, simultaneous selection for the plasmid-coded drug resistance and for the inactivation of the complementing gene directly yields colonies, which harbor recombinant plasmids. It is demonstrated that this cloning system can be used with an efficiency beyond 90%. Vector sequences are provided." @default.
• W2062516474 created "2016-06-24" @default.
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• W2062516474 date "1987-08-01" @default.
• W2062516474 modified "2023-09-24" @default.
• W2062516474 title "Direct selection of recombinant plasmids with chlorate" @default.
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• W2062516474 doi "https://doi.org/10.1111/j.1574-6968.1987.tb02123.x" @default.
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