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• W206263215 abstract "Research Article1 February 1984free access Isolation and characterization of the human prolactin gene. A.T. Truong A.T. Truong Search for more papers by this author C. Duez C. Duez Search for more papers by this author A. Belayew A. Belayew Search for more papers by this author A. Renard A. Renard Search for more papers by this author R. Pictet R. Pictet Search for more papers by this author G.I. Bell G.I. Bell Search for more papers by this author J.A. Martial J.A. Martial Search for more papers by this author A.T. Truong A.T. Truong Search for more papers by this author C. Duez C. Duez Search for more papers by this author A. Belayew A. Belayew Search for more papers by this author A. Renard A. Renard Search for more papers by this author R. Pictet R. Pictet Search for more papers by this author G.I. Bell G.I. Bell Search for more papers by this author J.A. Martial J.A. Martial Search for more papers by this author Author Information A.T. Truong, C. Duez, A. Belayew, A. Renard, R. Pictet, G.I. Bell and J.A. Martial The EMBO Journal (1984)3:429-437https://doi.org/10.1002/j.1460-2075.1984.tb01824.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Prolactin (PRL) and growth hormone (GH) genes derive from a common ancestor and still share some sequence homologies. Their expression in the pituitary gland is regulated in opposite directions by most of the many hormones acting on them. This provides an interesting system to study sequences involved in gene expression. Using a human PRL cDNA clone as a probe, we screened a human genomic DNA library in lambda phage and isolated a single recombinant comprising the whole hPRL gene. It was characterized by restriction endonuclease mapping and cDNA hybridization, by DNA heteroduplex analysis and by nucleotide sequencing. The hPRL gene is present as a single copy per haploid genome, is approximately 10 kb long and contains four introns, three of which interrupt the coding sequence at the same locations as in the known GH and PRL genes. The origin of transcription was determined by S1 mapping on prolactinoma mRNAs. The search for direct and inverted repeats, as well as dyad symmetries was carried out in the 900-bp sequenced in the 5′-flanking region. Sequence homologies between hPRL, hGH and rPRL were derived from computer drawn matrices for these upstream regions. Previous ArticleNext Article Volume 3Issue 21 February 1984In this issue RelatedDetailsLoading ..." @default.
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• W206263215 title "Isolation and characterization of the human prolactin gene." @default.
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