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- W2062641992 abstract "Molecules of DNA isolated from bacteriophage λ break in half, but no further, when subjected to the appropriate amount of hydrodynamic shear. The half-molecules can be separated from whole molecules by zone sedimentation in a sucrose gradient. The phage particles produced by bacteria exposed to λ DNA and active “helper” phage reveal the genes carried into the bacterium by the DNA. Whole molecules of λ DNA appear to be the entire phage chromosome since they carry genes from all parts of the recombination map: m6, m5, h, s, i and mi. One of the two half-molecules produced by shear breakage of λ DNA is capable of transferring s, i and mi, but not m6, m5 or h. Since this incomplete complement is a continuous segment of the linkage map of λ, it is argued that the sequence of genes on the recombination map is the same as the sequence of the nucleotides or blocks of nucleotides which correspond to each of the genes in a molecule of λ DNA." @default.
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- W2062641992 title "The production of phage chromosome fragments and their capacity for genetic transfer" @default.
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- W2062641992 doi "https://doi.org/10.1016/s0022-2836(62)80005-9" @default.
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